Department of Pharmacy, Zhangjiakou City First Hospital, Zhangjiakou, China.
Iran J Kidney Dis. 2023 May;17(3):135-140.
The research was an attempt to explore the potential impact of allicin on lipid peroxidation and oxidative stress in rats diagnosed with chronic kidney disease (CKD), and to determine its underlying mechanism.
Sixty rats were randomly divided into sham-operated, modelling, and allicin low, medium, and high dose groups. The histopathological structure of the kidney was observed in each group. Biochemical measurements were conducted to assess kidney function, including serum creatinine (Scr) and blood urea nitrogen (BUN), and 24-hour urine protein quantification. Levels of malondialdehyde (MDA), superoxide dismutase (SOD), reactive oxidative species (ROS), and reduced glutathione (GSH) in kidney tissue were measured, and mitogen-activated protein kinase (MAPK) and NF (nuclear factor) -κB protein levels were detected by western blotting.
They showed that allicin improved the pathological structure of renal tissue and protected renal function by reducing oxidative stress and lipid peroxidation via targeting the ROS/ MAPK/NF-κB pathway. Allicin increased SOD and GSH levels, while decreasing Scr, MDA, ROS, BUN, and the amount of protein excreted in urine over a 24-hour in medium and high dose groups. MAPK and NF-κB protein levels in medium and high dose allicin groups were lower than the modelling group.
Based on the results, it can be inferred that allicin may safeguard renal function in rats with CKD and has the potential to serve as a treatment for kidney ailments. DOI: 10.52547/ijkd.7496.
本研究旨在探讨大蒜素对慢性肾脏病（CKD）大鼠脂质过氧化和氧化应激的潜在影响，并探讨其作用机制。
将 60 只大鼠随机分为假手术组、模型组和大蒜素低、中、高剂量组。观察各组大鼠肾脏的组织病理学结构。进行生化指标检测，评估肾功能，包括血清肌酐（Scr）和血尿素氮（BUN），并进行 24 小时尿蛋白定量。测量肾组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、活性氧（ROS）和还原型谷胱甘肽（GSH）的水平，并通过 Western blot 检测丝裂原激活蛋白激酶（MAPK）和核因子（NF）-κB 蛋白水平。
大蒜素通过靶向 ROS/MAPK/NF-κB 通路，改善肾脏组织的病理结构，减轻氧化应激和脂质过氧化，从而保护肾功能。大蒜素可提高 SOD 和 GSH 水平，降低 Scr、MDA、ROS、BUN 和高、中剂量组大鼠 24 小时尿蛋白排泄量。中、高剂量大蒜素组 MAPK 和 NF-κB 蛋白水平低于模型组。
基于这些结果，可以推断大蒜素可能对 CKD 大鼠的肾功能具有保护作用，并有潜力成为肾脏疾病的治疗方法。DOI: 10.52547/ijkd.7496.
