Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Biomater Sci. 2023 Jul 25;11(15):5232-5239. doi: 10.1039/d3bm00308f.
Piezoelectric polymer nanofibers are attracting increasing attention in the stimulation of cell growth and proliferation in tissue engineering and wound healing applications. However, their intrinsic non-biodegradability hinders widespread applications in the biological fields. Herein, we designed, synthesized and characterized composite materials of silk fibroin (SF)/LiNbO (LN) nanoparticles/MWCNTs by electrospinning technology, which displayed good biocompatibility and comparable piezoelectric properties with an output current of up to 15 nA and output voltage of up to 0.6 V under pressure stimulation, remaining stable after 200 cycles of pressure release without significant decay. Meanwhile, the mechanical properties of the LN/CNTs/SF-nanofiber scaffolds (SF-NFSs) are also enhanced, with a tensile strength reaching 12.84 MPa and an elongation at break reaching 80.07%. Importantly, cell proliferation experiments showed that the LN/CNTs/SF-NFSs promoted cell proliferation at a rate of 43%. Accordingly, the mouse wound healing experiments further indicated that they could accelerate the healing of skin wounds in mice that were continuously moving. Therefore, SF-based piezoelectric nanofibrous scaffolds exhibit potential for use in rapid wound healing and this sheds light on smart treatment for tissue engineering in biomedicine.
压电聚合物纳米纤维在组织工程和伤口愈合应用中刺激细胞生长和增殖方面受到越来越多的关注。然而，其内在的不可生物降解性阻碍了它们在生物领域的广泛应用。在此，我们通过静电纺丝技术设计、合成和表征了丝素蛋白（SF）/LiNbO（LN）纳米粒子/MWCNTs 的复合材料，其表现出良好的生物相容性和可比拟的压电性能，在压力刺激下可产生高达 15nA 的输出电流和高达 0.6V 的输出电压，在 200 次压力释放循环后仍保持稳定，没有明显衰减。同时，LN/CNTs/SF-纳米纤维支架（SF-NFSs）的机械性能也得到了增强，拉伸强度达到 12.84MPa，断裂伸长率达到 80.07%。重要的是，细胞增殖实验表明 LN/CNTs/SF-NFSs 可将细胞增殖率提高到 43%。相应地，小鼠伤口愈合实验进一步表明，它们可以加速持续运动的小鼠皮肤伤口的愈合。因此，基于 SF 的压电纳米纤维支架在快速伤口愈合方面具有应用潜力，这为生物医学中的组织工程智能治疗提供了新的思路。
