Inorganic and Physical Chemistry Laboratory, Council of Scientific and Industrial Research-Central Leather Research Institute, Chennai, India.
Biochemistry and Biotechnology Laboratory, Council of Scientific and Industrial Research-Central Leather Research Institute, Chennai, India.
J Biomater Sci Polym Ed. 2023 Jan;34(1):35-52. doi: 10.1080/09205063.2022.2106707. Epub 2022 Aug 5.
Silk fibroin nanofibers find broader applications in skin tissue engineering as wound dressings. In this study, we have prepared biocompatible collagen-coated silk fibroin nanofibers with two small molecules: sinomenine hydrochloride (SH) and kaempferol hydrate (KH) with bioactive properties for wound healing applications. The prepared nanofibrous scaffolds were characterized different experimental techniques and the biocompatibility of the nanofibrous scaffolds was assessed using MTT assay and live/dead cell assay. The wound healing potential of the nanofibrous scaffolds was evaluated through animal model. Notably, the collagen-coated scaffolds showed improved biocompatibility and fibroblast viability over the uncoated scaffolds. The collagen-coated silk nanofibers containing KH showed good antioxidant properties and promoted wound healing in studies by minimizing inflammation and enhancing collagen deposition. Thus, the incorporation of antioxidant molecules along with collagen coating enhanced the wound healing efficiency of silk nanofibers.
丝素纳米纤维作为伤口敷料在皮肤组织工程中有更广泛的应用。在这项研究中，我们制备了具有生物相容性的胶原涂层丝素纳米纤维，其中含有两种具有生物活性的小分子：盐酸青藤碱（SH）和山奈酚水合物（KH），可用于伤口愈合应用。通过不同的实验技术对制备的纳米纤维支架进行了表征，并通过 MTT 测定法和活/死细胞测定法评估了纳米纤维支架的生物相容性。通过动物模型评估了纳米纤维支架的伤口愈合潜力。值得注意的是，与未涂层支架相比，胶原涂层支架显示出更好的生物相容性和成纤维细胞活力。含有 KH 的胶原涂层丝纳米纤维具有良好的抗氧化性能，并通过最小化炎症和增强胶原蛋白沉积来促进伤口愈合。因此，抗氧化分子的掺入以及胶原涂层的增加提高了丝纳米纤维的伤口愈合效率。
