Christensen P, Burova L A, Grubb A, Grubb R, Samuelsson G, Schalén C, Svensson M L
Acta Pathol Microbiol Scand C. 1979 Feb;87C(1):73-7.
Lancefield extracts of 19 types of group A streptococci as well as one group C and one group G strain were examined for agglutination of human red cells coated with various anti-Rh antibodies. Fourteen extracts agglutinated one or more of the coated cell samples, while five did not. The agglutination was inhibited by Fc but not by Fab fragments of human IgG. After mouse passages, three of the non-agglutinating strains acquired agglutinating capacity. At least three different reactivities were distinguished by the action of the extracts on IgG1 and IgG3 coated cells, respectively. Two of the streptococcal extracts, agglutinating the same anti-Rh coated cells, could be further differentiated in hemagglutination inhibition (HAI) experiments using purified IgG3 myeloma proteins. Five selected agglutinating systems were inhibited by purified myeloma proteins of the IgG1, IgG2, and IgG4 subclasses. IgG3 proteins inhibited only two of the five HAI systems.
对19种A组链球菌以及1株C组和1株G组菌株的兰斯菲尔德提取物进行了检测，以观察其对包被有各种抗Rh抗体的人红细胞的凝集作用。14种提取物能凝集一种或多种包被细胞样本，而5种则不能。凝集作用被人IgG的Fc片段抑制，但不被Fab片段抑制。经小鼠传代后，3种非凝集菌株获得了凝集能力。通过提取物分别对IgG1和IgG3包被细胞的作用，至少区分出三种不同的反应性。两种能凝集相同抗Rh包被细胞的链球菌提取物，在使用纯化的IgG3骨髓瘤蛋白进行的血凝抑制（HAI）实验中可进一步区分。5个选定的凝集系统被IgG1、IgG2和IgG4亚类的纯化骨髓瘤蛋白抑制。IgG3蛋白仅抑制5个HAI系统中的2个。
