Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2023 Apr;509(1):47-50. doi: 10.1134/S1607672922600208. Epub 2023 Jun 20.
The Drosophila transcription factor СР190 is one of the key proteins that determine the activity of housekeeping gene promoters and insulators. CP190 has an N-terminal BTB domain that allows for dimerization. Many of known Drosophila architectural proteins interact with the hydrophobic peptide-binding groove in the BTB domain, which is presumably a mechanisms for recruiting CP190 to regulatory elements. To study the role of the BTB domain in the interaction with architectural proteins, we obtained transgenic flies expressing CP190 variants with mutations in the peptide-binding groove, which disrupts their interaction with architectural proteins. As a result of the studies, it was found that mutations in the BTB domain do not affect binding of the CP190 protein to polytene chromosomes. Thus, our studies confirm the previously obtained data that CP190 is recruited to regulatory elements by several transcription factors interacting, in addition to BTB, with other CP190 domains.
果蝇转录因子СР190 是决定管家基因启动子和绝缘子活性的关键蛋白之一。CP190 具有一个允许二聚化的 N 端 BTB 结构域。许多已知的果蝇结构蛋白与 BTB 结构域中的疏水性肽结合槽相互作用，这可能是将 CP190 募集到调节元件的机制。为了研究 BTB 结构域在与结构蛋白相互作用中的作用，我们获得了表达 CP190 变体的转基因果蝇，这些变体在肽结合槽中发生突变，从而破坏了它们与结构蛋白的相互作用。研究结果发现，BTB 结构域中的突变不影响 CP190 蛋白与多线染色体的结合。因此，我们的研究证实了之前获得的数据，即 CP190 除了 BTB 结构域外，还通过与其他 CP190 结构域相互作用的几个转录因子被募集到调节元件。
