State Key Laboratory of Systems Medicine for Cancer, School of biomedical engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Institute of Medical Robotics, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.
Adv Sci (Weinh). 2023 Aug;10(24):e2301721. doi: 10.1002/advs.202301721. Epub 2023 Jun 20.
Non-invasive detection and precise localization of deep lesions have attracted significant attention for both fundamental and clinical studies. Optical modality techniques are promising with high sensitivity and molecular specificity, but are limited by shallow tissue penetration and the failure to accurately determine lesion depth. Here the authors report in vivo ratiometric surface-enhanced transmission Raman spectroscopy (SETRS) for non-invasive localization and perioperative surgery navigation of deep sentinel lymph nodes in live rats. The SETRS system uses ultrabright surface-enhanced Raman spectroscopy (SERS) nanoparticles with a low detection limit of 10 pM and a home-built photosafe transmission Raman spectroscopy setup. The ratiometric SETRS strategy is proposed based on the ratio of multiple Raman spectral peaks for obtaining lesion depth. Via this strategy, the depth of the phantom lesions in ex vivo rat tissues is precisely determined with a mean-absolute-percentage-error of 11.8%, and the accurate localization of a 6-mm-deep rat popliteal lymph node is achieved. The feasibility of ratiometric SETRS allows the successful perioperative navigation of in vivo lymph node biopsy surgery in live rats under clinically safe laser irradiance. This study represents a significant step toward the clinical translation of TRS techniques, providing new insights for the design and implementation of in vivo SERS applications.
非侵入式检测和精确的深层病变定位引起了基础研究和临床研究的广泛关注。光学模式技术具有高灵敏度和分子特异性的优势，但受到组织浅层穿透深度的限制，并且无法准确确定病变深度。本文作者报道了一种用于活体大鼠深部前哨淋巴结的非侵入式定位和围手术期手术导航的比率型表面增强传输拉曼光谱（SETRS）技术。该 SETRS 系统使用超低检测限（10pM）的超亮表面增强拉曼散射（SERS）纳米粒子和自建的光安全传输拉曼光谱仪。该比率型 SETRS 策略基于多个拉曼光谱峰的比值来获取病变深度。通过该策略，离体大鼠组织中模拟病变的深度能够被精确地确定，平均绝对百分比误差为 11.8%，并且能够实现 6mm 深的大鼠腘淋巴结的准确定位。比率型 SETRS 的可行性允许在临床安全的激光辐照下对活体大鼠进行淋巴结活检手术的围手术期导航。本研究朝着 TRS 技术的临床转化迈出了重要一步，为体内 SERS 应用的设计和实施提供了新的思路。
