Department of Periodontology, Faculty of Dentistry, Nuh Naci Yazgan University, Kayseri, Turkey.
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Nuh Naci Yazgan University, Kayseri, Turkey.
J Periodontal Res. 2023 Oct;58(5):932-938. doi: 10.1111/jre.13151. Epub 2023 Jun 20.
Paeoniflorin (Pae) is a monoterpene glycoside with immune-regulatory effects. Several studies have already demonstrated the impact of Pae on periodontitis, but its effect on diabetic periodontitis is unclear. In this study, our aim was to test the hypothesis that Pae had a strong anti-inflammatory effect that prevented bone loss in diabetic periodontitis.
Thirty male Wistar albino rats were randomly divided into control (healthy, n = 10), periodontitis (PD) + diabetes (DM; n = 10), and PD + DM + Pae (n = 10) groups. Ligature-induced periodontitis was created by placing 4-0 silk ligatures around the lower first molars on both sides of the mandibulae. Experimental DM was created via an injection of 50 mg/kg and streptozotocin (STZ). Hyperglycemia was confirmed by the blood glucose levels of rats (>300 mg/dL). The bone mineral density (BMD), trabecular number, trabecular thickness, and bone loss were measured by micro-CT. The expression levels of IL-1β, IL-6, and TNF-α were measured in tissue homogenates by ELISA.
The PD + DM + Pae group had significantly less alveolar crest resorption when compared to the PD + DM group. There was also a significant difference between the PD + DM + Pae group compared to PD + DM group in trabecular thickness, BMD, and the number of trabeculae. Pae application led to a statistically significant decrease in IL-1β, IL-6, and TNF-α levels in diabetic periodontitis.
Systemic application of Pae suppressed inflammation caused by PD and DM, leading to reduced bone loss and enhanced bone quality.
芍药苷（Pae）是一种具有免疫调节作用的单萜糖苷。已有多项研究表明芍药苷对牙周炎有影响，但它对糖尿病性牙周炎的影响尚不清楚。在本研究中，我们的假设是芍药苷具有很强的抗炎作用，可以防止糖尿病性牙周炎中的骨丢失。
将 30 只雄性 Wistar 白化大鼠随机分为对照组（健康，n=10）、牙周炎（PD）+糖尿病（DM；n=10）和牙周炎（PD）+DM+芍药苷（Pae；n=10）组。在下颌骨双侧第一磨牙周围放置 4-0 丝线结扎，以建立结扎诱导的牙周炎。通过注射 50mg/kg 和链脲佐菌素（STZ）来建立实验性糖尿病。通过大鼠血糖水平（>300mg/dL）来确认高血糖。通过 micro-CT 测量骨矿物质密度（BMD）、小梁数、小梁厚度和骨丢失。通过 ELISA 测量组织匀浆中 IL-1β、IL-6 和 TNF-α 的表达水平。
与 PD+DM 组相比，PD+DM+Pae 组牙槽嵴吸收明显减少。与 PD+DM 组相比，PD+DM+Pae 组的小梁厚度、BMD 和小梁数也有显著差异。芍药苷的应用导致糖尿病性牙周炎中 IL-1β、IL-6 和 TNF-α 水平显著降低。
系统应用芍药苷抑制 PD 和 DM 引起的炎症，导致骨丢失减少和骨质量增强。
