Department of Veterinary Medicine, Federal University of Lavras (UFLA), Lavras 37200-000, Minas Gerais, Brazil.
Department of Health Sciences, Federal University of Lavras (UFLA), Lavras 37200-000, Minas Gerais, Brazil.
Nutrients. 2017 Sep 14;9(9):1016. doi: 10.3390/nu9091016.
This study aimed to evaluate the effects of β-glucan ingestion () on the plasmatic levels of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), alveolar bone loss, and pancreatic β-cell function (HOMA-BF) in diabetic rats with periodontal disease (PD). Besides, intestinal morphology was determined by the villus/crypt ratio. A total of 48 Wistar rats weighing 203 ± 18 g were used. Diabetes was induced by the intraperitoneal injection of streptozotocin (80 mg/kg) and periodontal inflammation, by ligature. The design was completely randomized in a factorial scheme 2 × 2 × 2 (diabetic or not, with or without periodontitis, and ingesting β-glucan or not). The animals received β-glucan by gavage for 28 days. Alveolar bone loss was determined by scanning electron microscopy (distance between the cementoenamel junction and alveolar bone crest) and histometric analysis (bone area between tooth roots). β-glucan reduced plasmatic levels of TNF-α in diabetic animals with PD and of IL-10 in animals with PD ( < 0.05). β-glucan reduced bone loss in animals with PD ( < 0.05). In diabetic animals, β-glucan improved β-cell function ( < 0.05). Diabetic animals had a higher villus/crypt ratio ( < 0.05). In conclusion, β-glucan ingestion reduced the systemic inflammatory profile, prevented alveolar bone loss, and improved β-cell function in diabetic animals with PD.
本研究旨在评估 β-葡聚糖摄入对伴牙周病（PD）糖尿病大鼠的肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）的血浆水平、牙槽骨丧失和胰岛β细胞功能（HOMA-BF）的影响。此外，还通过绒毛/隐窝比来确定肠道形态。共使用了 48 只体重为 203±18g 的 Wistar 大鼠。糖尿病通过腹腔注射链脲佐菌素（80mg/kg）和结扎诱导，牙周炎通过结扎诱导。设计采用完全随机分组的析因设计 2×2×2（是否糖尿病、是否伴牙周炎、是否摄入β-葡聚糖）。动物通过灌胃接受 β-葡聚糖 28 天。通过扫描电子显微镜（牙釉质-牙骨质交界处与牙槽嵴之间的距离）和组织学分析（牙根之间的骨面积）来确定牙槽骨丧失。β-葡聚糖降低了 PD 伴糖尿病动物的 TNF-α和 PD 动物的 IL-10 的血浆水平（<0.05）。β-葡聚糖降低了 PD 动物的骨丢失（<0.05）。在糖尿病动物中，β-葡聚糖改善了β细胞功能（<0.05）。糖尿病动物的绒毛/隐窝比更高（<0.05）。总之，β-葡聚糖摄入降低了伴 PD 的糖尿病动物的系统性炎症特征，预防了牙槽骨丧失，并改善了β细胞功能。
