College of Life Sciences, Xinjiang Agricultural University, Urumqi, 830052, China.
College of Agriculture, Xinjiang Agricultural University, Urumqi, 830052, China.
Plant Mol Biol. 2023 Jul;112(4-5):261-277. doi: 10.1007/s11103-023-01361-5. Epub 2023 Jun 21.
Haloxylon ammodendron, an important shrub utilized for afforestation in desert areas, can withstand harsh ecological conditions such as drought, high salt and extreme heat. A better understanding of the stress adaptation mechanisms of H. ammodendron is vital for ecological improvement in desert areas. In this study, the role of the H. ammodendron 14-3-3 protein HaFT-1 in thermotolerance was investigated. qRT-PCR analysis showed that heat stress (HS) priming (the first HS) enhanced the expression of HaFT-1 during the second HS and subsequent recovery phase. The subcellular localization of YFP-HaFT-1 fusion protein was mainly detected in cytoplasm. HaFT-1 overexpression increased the germination rate of transgenic Arabidopsis seeds, and the survival rate of HaFT-1 overexpression seedlings was higher than that of wild-type (WT) Arabidopsis after priming-and-triggering and non-primed control treatments. Cell death staining showed that HaFT-1 overexpression lines exhibited significantly reduced cell death during HS compared to WT. Transcriptome analysis showed that genes associated with energy generation, protein metabolism, proline metabolism, autophagy, chlorophyll metabolism and reactive oxygen species (ROS) scavenging were important to the thermotolerance of HS-primed HaFT-1 transgenic plants. Growth physiology analysis indicated that priming-and-triggering treatment of Arabidopsis seedlings overexpressing HaFT-1 increased proline content and strengthened ROS scavenging activity. These results demonstrated that overexpression of HaFT-1 increased not only HS priming but also tolerance to the second HS of transgenic Arabidopsis, suggesting that HaFT-1 is a positive regulator in acquired thermotolerance.
梭梭是一种重要的荒漠造林灌木，可以耐受干旱、高盐和极端高温等恶劣的生态条件。深入了解梭梭适应胁迫的机制对于荒漠生态的改善至关重要。本研究探讨了梭梭 14-3-3 蛋白 HaFT-1 在耐热性中的作用。qRT-PCR 分析表明，热激预处理（第一次热激）增强了 HaFT-1 在第二次热激和随后的恢复阶段的表达。YFP-HaFT-1 融合蛋白的亚细胞定位主要在细胞质中检测到。HaFT-1 过表达提高了转基因拟南芥种子的萌发率，并且在预处理和触发以及非预处理对照处理后，HaFT-1 过表达幼苗的存活率高于野生型（WT）拟南芥。细胞死亡染色表明，与 WT 相比，HaFT-1 过表达系在热激期间表现出明显减少的细胞死亡。转录组分析表明，与能量产生、蛋白质代谢、脯氨酸代谢、自噬、叶绿素代谢和活性氧（ROS）清除相关的基因对热激预处理 HaFT-1 转基因植物的耐热性很重要。生长生理分析表明，过表达 HaFT-1 的拟南芥幼苗的预处理和触发处理增加了脯氨酸含量并增强了 ROS 清除活性。这些结果表明，HaFT-1 的过表达不仅增加了热激预处理，而且提高了转基因拟南芥对第二次热激的耐受性，表明 HaFT-1 是获得耐热性的正调控因子。
