Lee Chang-Min, Park Hyun-Su, Baek Man-Kee, Jeong O-Young, Seo Jeonghwan, Kim Suk-Man
Crop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju, Republic of Korea.
Department of Ecological & Environmental System, Kyungpook National University, Sangju, Republic of Korea.
Front Plant Sci. 2023 Jun 5;14:1194058. doi: 10.3389/fpls.2023.1194058. eCollection 2023.
The stability of cultivation and production in terms of crop yield has been threatened by climate change due to global warming. Pre-harvest sprouting (PHS) is a threat to crops, especially staple foods, including rice, because of reductions in yield and quality. To address the problem of precocious germination before harvest, we performed quantitative trait loci (QTL) analysis for PHS using F RILs populations derived from weedy rice in Korea. QTL analysis revealed that two stable QTLs, and , associated with PHS resistance were identified on chromosomes 7 and 2, respectively, explaining approximately 38% of the phenotypic variation. The QTL effect in the tested lines significantly decreased the degree of PHS, based on the number of QTLs included. Through fine mapping for main QTL , the region for the PHS was found to be anchored within 23.575-23.785 Mbp on chromosome 7 using 13 cleaved amplified sequence (CAPS) markers. Among 15 open reading frames (ORFs) within the detected region, one ORF, Os07g0584366, exhibited upregulated expression in the resistant donor, which was approximately nine times higher than that of susceptible cultivars under PHS-inducing conditions. lines with QTLs related to PHS resistance were developed to improve the characteristics of PHS and design practical PCR-based DNA markers for marker-assisted backcrosses of many other PHS-susceptible cultivars.
由于全球变暖导致的气候变化，作物产量方面的种植和生产稳定性受到了威胁。收获前发芽（PHS）对作物，尤其是包括水稻在内的主食作物构成了威胁，因为它会导致产量和品质下降。为了解决收获前过早发芽的问题，我们利用源自韩国杂草稻的F RILs群体对PHS进行了数量性状位点（QTL）分析。QTL分析表明，分别在第7号和第2号染色体上鉴定出两个与PHS抗性相关的稳定QTL，即 和 ，它们解释了约38%的表型变异。根据所包含的QTL数量，测试品系中的QTL效应显著降低了PHS的程度。通过对主要QTL 的精细定位，利用13个酶切扩增多态性序列（CAPS）标记，发现PHS区域定位于第7号染色体上23.575 - 23.785 Mbp之间。在检测区域内的15个开放阅读框（ORF）中，一个ORF，即Os07g0584366，在抗性供体中表现出上调表达，在PHS诱导条件下，其表达量比易感 品种高出约9倍。开发了具有与PHS抗性相关QTL的 品系，以改善PHS特性，并设计基于PCR的实用DNA标记，用于许多其他PHS易感 品种的标记辅助回交。
