College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, China.
Sci Rep. 2024 Nov 18;14(1):28485. doi: 10.1038/s41598-024-79474-0.
FAR-RED IMPAIRED RESPONSE 1 (FAR1) is a class of transposase-derived transcription factors that play a very important role in the initiation of the photosensitive pigment A (phyA) signaling pathway. Despite their importance, the understanding of the function of FAR1 genes in quinoa is still limited, especially regarding how they affect the spike sprouting response. Quinoa has gained global attention in recent years for its health benefits and potential for sustainable agriculture. In our study, the CqFAR1 gene set in quinoa was characterized using HMMER (PF03101) and BLAST analyses, and 87 genes were identified. The 87 CqFAR1 genes were systematically classified into five groups that showed a high degree of conservation in gene structure and motif composition. Tissue expression profiles of the CqFAR1 gene indicated that the CqFAR1 gene plays a key role throughout the growth and development of quinoa, especially at mid (leaf) and end (spike) stages. By RT-qPCR analysis, we observed significant differences in the expression of the CqFAR1 gene at different developmental stages. Notably, the CqFAR1 gene showed significant expression enhancement at the early stage of quinoa spike sprouting. The results are useful for understanding the role of the CqFAR1 gene in quinoa growth and development and provide theoretical support for quinoa breeding.
远红光受抑响应 1（FAR1）是一类转座酶衍生的转录因子，在光敏色素 A（phyA）信号通路的起始中发挥着非常重要的作用。尽管它们很重要，但 FAR1 基因在藜麦中的功能仍然知之甚少，特别是它们如何影响穗发芽反应。近年来，藜麦因其健康益处和可持续农业的潜力而引起了全球关注。在我们的研究中，使用 HMMER（PF03101）和 BLAST 分析对藜麦中的 CqFAR1 基因进行了表征，鉴定出了 87 个基因。这 87 个 CqFAR1 基因被系统地分为五个组，它们在基因结构和基序组成上具有高度的保守性。CqFAR1 基因的组织表达谱表明，该基因在藜麦的生长和发育过程中起着关键作用，尤其是在中期（叶片）和末期（穗）阶段。通过 RT-qPCR 分析，我们观察到 CqFAR1 基因在不同发育阶段的表达存在显著差异。值得注意的是，CqFAR1 基因在藜麦穗发芽的早期表现出显著的表达增强。这些结果有助于理解 CqFAR1 基因在藜麦生长和发育中的作用，并为藜麦的培育提供理论支持。
