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优化感染性 EcoHIV 病毒粒子生产和质量控制的方案。

Protocol for optimizing production and quality control of infective EcoHIV virions.

机构信息

University of Kentucky, College of Medicine, Lexington, KY 40506, USA.

Massachusetts Institute of Technology, Division of Comparative Medicine, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.

出版信息

STAR Protoc. 2023 Sep 15;4(3):102368. doi: 10.1016/j.xpro.2023.102368. Epub 2023 Jun 19.

DOI:10.1016/j.xpro.2023.102368
PMID:37342907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10511864/
Abstract

EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

摘要

生态 HIV 是一种 HIV 感染模型，可在小鼠中重现 HIV-1 病理学的某些方面。然而，目前发表的指导 EcoHIV 病毒粒子生产的方案有限。在这里，我们提出了一种生产感染性 EcoHIV 病毒粒子和必要质量控制的方案。我们描述了病毒纯化、滴度测定和多种分析感染功效的技术步骤。该方案可在 C57BL/6 小鼠中产生高感染性，这将有助于研究人员生成临床前数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/ab4200095804/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/d6f3ae5df6f7/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/8874ee015983/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/aa160f162a2f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/e6e0827bcbd4/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/226ca9c03350/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/ed421a01bb93/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/3c40ddb01b08/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/ab4200095804/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/d6f3ae5df6f7/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/8874ee015983/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/aa160f162a2f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/e6e0827bcbd4/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/226ca9c03350/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/ed421a01bb93/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/3c40ddb01b08/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cae/10511864/ab4200095804/gr7.jpg

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