Chen Xin, Liu Ruize, Wang Wei, Liu Yundan, Sun Jianhua, Shao Hongbing, McMinn Andrew, Wang Min, Liang Yantao
College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, MOE Key Laboratory of Evolution and Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Center for Ocean Carbon Neutrality, Ocean University of China, Qingdao, PR China.
Department of Intensive Care Unit, Qingdao Hospital, University of Health and Rehabilitation Sciences (Qingdao Municipal Hospital), Qingdao, PR China.
Microb Genom. 2025 Apr;11(4). doi: 10.1099/mgen.0.001403.
are widely distributed in aquatic environments and are major pathogens commonly found in aquaculture environments, playing a significant role in human production activities and maintaining ecological stability. Here, a novel phage, vB_VneS_J26, which infects , was isolated from coastal seawater in Qingdao, China. Transmission electron microscopy revealed that vB_VneS_J26 exhibits siphovirus morphotype, with a linear double-stranded DNA genome of 82,477 bp in length and G+C content of 45.11 mol%, encoding 122 putative ORFs. Three auxiliary metabolic genes related to carbon metabolism and host cell redox processes were identified, including a pyruvate phosphate dikinase, which catalyses the reversible conversion between phosphoenolpyruvate and pyruvate and is rarely detected in viruses. Whole-genome phylogenetic and comparative genomic analyses suggested that vB_VneS_J26 represents a potential novel viral family, comprising six isolated vibriophages, proposed as . Phylogeographic analysis indicated that is primarily distributed in epipelagic and mesopelagic zones of the Arctic and temperate tropical oceans.
它们广泛分布于水生环境中，是水产养殖环境中常见的主要病原体，在人类生产活动和维持生态稳定方面发挥着重要作用。在此，从中国青岛的沿海海水中分离出一种新型噬菌体vB_VneS_J26，它可感染[此处原文缺失被感染对象]。透射电子显微镜显示，vB_VneS_J26呈现出肌尾噬菌体形态类型，其线性双链DNA基因组长度为82477 bp，G+C含量为45.11 mol%，编码122个推定的开放阅读框。鉴定出了三个与碳代谢和宿主细胞氧化还原过程相关的辅助代谢基因，包括一种磷酸丙酮酸双激酶，它催化磷酸烯醇丙酮酸和丙酮酸之间的可逆转化，在病毒中很少被检测到。全基因组系统发育和比较基因组分析表明，vB_VneS_J26代表一个潜在的新型病毒家族，包含六个分离的弧菌噬菌体，被提议命名为[此处原文缺失提议的名称]。系统地理分析表明，[此处原文缺失相关对象]主要分布在北极和温带热带海洋的上层和中层带。
