基于微流控的单细胞或液滴操控：方法与应用。

Microfluidic based single cell or droplet manipulation: Methods and applications.

机构信息

State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China.

State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.

出版信息

Talanta. 2023 Dec 1;265:124776. doi: 10.1016/j.talanta.2023.124776. Epub 2023 Jun 15.

DOI:10.1016/j.talanta.2023.124776

PMID:37348357

Abstract

The isolation of single cell or droplet is first and crucial step to single-cell analysis, which is important for cancer research and diagnostic methods. This review provides an overview of technologies that are currently used or in development to realize the isolation. Microfluidic based manipulation is an emerging technology with the distinct advantages of miniaturization and low cost. Therefore, recent developments in microfluidic isolated methods have attracted extensive attention. We introduced herein five strategies based on microfluid: trap, microfluidic discrete manipulation, bioprinter, capillary and inertial force. For every technology, their basic principles and features were discussed firstly. Then some modified approaches and applications were listed as the extension. Finally, we compared the advantages and drawbacks of these methods, and analyzed the trend of the manipulation based on microfluidics.

摘要

单细胞或液滴的分离是单细胞分析的第一步和关键步骤，这对于癌症研究和诊断方法很重要。本文综述了目前用于或正在开发用于实现分离的技术。基于微流控的操作是一种新兴技术，具有微型化和低成本的明显优势。因此，微流控分离方法的最新进展引起了广泛关注。我们在这里介绍了基于微流控的五种策略：捕获、微流离散操作、生物打印、毛细管和惯性力。对于每种技术，首先讨论了它们的基本原理和特点。然后列出了一些改进的方法和应用作为扩展。最后，我们比较了这些方法的优缺点，并分析了基于微流控的操作趋势。