从巨型锥虫的上鞭毛体形式中分离和鉴定一种中性糖鞘脂。

Isolation and characterization of a neutral glycosphingolipid from the epimastigote form of Trypanosoma mega.

作者信息

Vermelho A B, Hogge L, Barreto-Bergter E

出版信息

J Protozool. 1986 May;33(2):208-13. doi: 10.1111/j.1550-7408.1986.tb05591.x.

DOI:10.1111/j.1550-7408.1986.tb05591.x

Abstract

A glycosphingolipid fraction from Trypanosoma mega was isolated after acetylation and was further purified on a silicic acid column. Final purification was by preparative thin-layer chromatography. The carbohydrate components of the glycolipid were fucose and galactose in approximately equimolar amounts. The neutral glycolipid of T. mega has a sphingosine base composition that consists of sphingosine and traces of dihydrosphingosine. Fatty acids forming amide groups with the sphingosine bases were analyzed by gas-liquid chromatography-mass spectrometry and are a mixture of normal and alpha-hydroxy fatty acids. Normal C16:0, C18:0, and 2-hydroxy C18:0 are the predominant fatty acids.

摘要

大型锥虫的糖鞘脂部分经乙酰化后分离出来，并在硅酸柱上进一步纯化。最终通过制备型薄层色谱法进行纯化。该糖脂的碳水化合物成分是岩藻糖和半乳糖，含量大致等摩尔。大型锥虫的中性糖脂具有由鞘氨醇和痕量二氢鞘氨醇组成的鞘氨醇碱基组成。通过气液色谱 - 质谱联用分析与鞘氨醇碱基形成酰胺基团的脂肪酸，它们是正脂肪酸和α - 羟基脂肪酸的混合物。主要脂肪酸是正C16:0、C18:0和2 - 羟基C18:0。