Yue B Y, Tao R V, Lee B C, Sugar J
Department of Ophthalmology, Lions of Illinois Eye Research Institute, University of Illinois, Chicago College of Medicine 60612.
Curr Eye Res. 1990 Apr;9(4):337-42. doi: 10.3109/02713689008999621.
Six neutral glycosphingolipids were isolated from porcine corneas using silicic acid column chromatography and preparative thin-layer chromatography. Five of these glycolipids were partially identified by gas-liquid chromatography. Two were glucosylceramides, two were lactosylceramides and one was tetrahexosylceramide containing galactose, glucose and N-acetylgalactosamine in the molar ratio of 2:1:1. Glucosylceramides were found to be the predominating component, with lactosyl- and tetrahexosylceramides being the minor constituents. Sphingosine was the major long-chain base in all fractions. The fatty acids of the corneal neutral glycosphingolipids were variable in chain length. This represents the first investigation of neutral glycosphingolipids in corneas of any species.
采用硅酸柱色谱法和制备型薄层色谱法从猪角膜中分离出六种中性糖鞘脂。其中五种糖脂通过气液色谱法进行了部分鉴定。两种是葡糖神经酰胺，两种是乳糖神经酰胺，一种是四己糖神经酰胺，其半乳糖、葡萄糖和N-乙酰半乳糖胺的摩尔比为2:1:1。发现葡糖神经酰胺是主要成分，乳糖神经酰胺和四己糖神经酰胺是次要成分。鞘氨醇是所有组分中的主要长链碱。角膜中性糖鞘脂的脂肪酸链长度各不相同。这是对任何物种角膜中中性糖鞘脂的首次研究。
