Ogiso M, Irie A, Kubo H, Komoto M, Matsuno T, Koide Y, Hoshi M
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
J Biol Chem. 1993 Jun 25;268(18):13242-7.
Neutral glycosphingolipids were purified from human senile cataractous lenses by a combination of solvent extraction, Folch's partition, acetylation, and column chromatography using DEAE-Sephadex and Iatrobeads. Six major glycosphingolipids (A-F) from monohexosylceramide to pentahexosylceramide were identified by sugar composition analysis, methylation analysis, secondary ion-mass spectrometry, glycosidase digestion, and chromium trioxide oxidation. Their structures suggested that they were closely related in their metabolism: their sugar chains were in sequence and their ceramide moieties were similarly composed, namely C16:0 and C24:1 constituted most of the fatty acids, and long-chain base components were mostly C18-dihydrosphingosine with a small portion of C18-sphingosine. The sugar chains implied two pathways branching from lactosylceramide: one to globotriaosylceramide and the other to lactotriaosylceramide, which leads to the production of Le(x) glycolipid via neolacto type 2 core chain.
通过溶剂萃取、福尔克分配、乙酰化以及使用二乙氨基乙基葡聚糖（DEAE - Sephadex）和离子交换树脂珠（Iatrobeads）的柱色谱法相结合，从人老年性白内障晶状体中纯化出中性糖鞘脂。通过糖组成分析、甲基化分析、二次离子质谱、糖苷酶消化和三氧化铬氧化，鉴定出六种主要的糖鞘脂（A - F），其范围从单己糖神经酰胺到五己糖神经酰胺。它们的结构表明它们在代谢上密切相关：它们的糖链依次排列，并且它们的神经酰胺部分组成相似，即十六烷酸（C16:0）和二十四碳烯酸（C24:1）构成了大部分脂肪酸，长链碱基成分主要是C18 - 二氢鞘氨醇，还有一小部分C18 - 鞘氨醇。糖链暗示了两条从乳糖神经酰胺分支的途径：一条通向球三糖神经酰胺，另一条通向乳糖三糖神经酰胺，后者通过新乳糖II型核心链导致Le(x)糖脂的产生。
