CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
Department of Biochemistry-Genetic, School of Biological Sciences, Université Peleforo Gon Coulibaly, Korhogo, Côte d'Ivoire.
Arch Virol. 2023 Jun 23;168(7):187. doi: 10.1007/s00705-023-05817-3.
The Acinetobacter baumannii bacteriophage Aci01-1, which belongs to the genus Saclayvirus of the order Caudoviricetes, has an icosahedral head and a contractile rigid tail. We report that Aci01-1 has, attached to the tail conical tip, a remarkable 146-nm-long flexible fiber with seven beads and a terminal knot. Its putative gene coding for a 241.36-kDa tail fiber protein is homologous to genes in Aci01-1-related and unrelated phages. Analysis of its 3D structure using AlphaFold provides a structural model for the fiber observed by electron microscopy. We also identified a putative receptor of the phage on the bacterial capsule that is hypothesized to interact with the Aci01-1 long fiber.
鲍曼不动杆菌噬菌体 Aci01-1 属于长尾病毒目 Saclayvirus 属，具有二十面体头部和可收缩的刚性尾部。我们报告称，Aci01-1 的尾部锥形尖端附着有一条长 146nm 的显著柔性纤维，其上有七个珠子和一个末端结。其编码 241.36kDa 尾丝蛋白的假定基因与 Aci01-1 相关和不相关噬菌体的基因同源。使用 AlphaFold 对其 3D 结构进行分析，为电镜观察到的纤维提供了结构模型。我们还鉴定了细菌荚膜上噬菌体的假定受体，该受体被假设与 Aci01-1 的长纤维相互作用。
