Terpstra W J, Schoone G J, Ligthart G S, ter Schegget J
Royal Tropical Institute, Department of Tropical Hygiene, Amsterdam, The Netherlands.
J Gen Microbiol. 1987 Apr;133(4):911-4. doi: 10.1099/00221287-133-4-911.
In situ DNA hybridization using biotin-labelled leptospiral DNA was performed on clinical specimens to investigate its usefulness as a technique for the identification of Leptospira interrogans. The applicability of this test in blood, urine and liver smears was demonstrated. In situ DNA hybridization can be completed in only 4 h and it combines the advantage of visualization of the leptospiral morphology with the specificity of the hybridization reaction. No cross-hybridization was observed with other bacteria. This study shows that hybridization in situ can be simple to perform and may contribute to a rapid diagnosis.
使用生物素标记的钩端螺旋体DNA对临床标本进行原位DNA杂交，以研究其作为问号钩端螺旋体鉴定技术的实用性。证明了该试验在血液、尿液和肝脏涂片上的适用性。原位DNA杂交仅需4小时即可完成，它将钩端螺旋体形态可视化的优势与杂交反应的特异性结合在一起。未观察到与其他细菌的交叉杂交。本研究表明，原位杂交操作简便，可能有助于快速诊断。
