Department of Horticulture, National Chiayi University, Chiayi 60004, Taiwan.
Department of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan 701401, Taiwan.
Food Chem. 2023 Nov 30;427:136685. doi: 10.1016/j.foodchem.2023.136685. Epub 2023 Jun 21.
Lactobacillus species confer health benefits by their metabolites, secreted molecules, and population numbers. Extracellular vesicles (EVs) are nano-sized particles released from cells and mediate intercellular communications. EVs-encapsulated cargos are a crucial key to decide involved biological function. However, little is known about the composition of EVs, leaving mechanisms by which Lactobacillus-derived EVs affect recipient cells remaining unresolved. This study examined the composition of EV proteins from Lactobacillus species by using liquid chromatography coupled with tandem mass spectrometry, including L. plantarum, L. fermentum, and L. gasseri. The major proteins of EVs are associated with biological processes such as catalytic activity, gluco-neogenesis, cell wall organization, and glycolytic processes. Motif enrichment analysis revealed that EVs from L. plantarum and L. fermentum contained proteins with serine-rich motif. This is the first study to report the composition and comparison of EV proteins from Lactobacillus species, providing important information of EVs in functional food products development.
乳酸菌通过其代谢产物、分泌分子和种群数量赋予健康益处。细胞外囊泡 (EVs) 是从细胞中释放的纳米大小的颗粒，介导细胞间通讯。EVs 包裹的货物是决定相关生物学功能的关键。然而，对于 EVs 的组成知之甚少，这使得乳酸菌衍生的 EVs 影响受体细胞的机制仍未解决。本研究使用液相色谱串联质谱法检查了包括植物乳杆菌、发酵乳杆菌和格氏乳杆菌在内的乳酸菌 EV 蛋白的组成。EV 的主要蛋白与生物过程相关，如催化活性、葡糖新生、细胞壁组织和糖酵解过程。基序富集分析表明，植物乳杆菌和发酵乳杆菌的 EV 中含有富含丝氨酸的蛋白。这是首次报道乳酸菌 EV 蛋白的组成和比较的研究，为功能性食品产品开发中的 EV 提供了重要信息。
