Park Seoah, Mok Jongsoo, Yu Hye-Min, An Hye-Jin, Choi Ga-Hyun, Lee Yeon-Seon, Kwon Ki-Jin, Choi Sung-Jun, Kim Kyung-Hee, Kim Soo-Jin, Park Joonghoon
Graduate School of International Agricultural Technology, Seoul National University, Seoul, Korea.
Schofield Biome Research Lab, HK inno. N, Koreasu, Seoul, Korea.
Sci Rep. 2025 Jul 26;15(1):27263. doi: 10.1038/s41598-025-12873-z.
Bacterial extracellular vesicles (bEVs) have therapeutic potential by mimicking the effects of the microbiome. Here, we characterized bEVs from eight gram-positive Lactobacillales strains, evaluating their therapeutic potential. In primary characterization, Lactobacillus paracasei produced the largest bEVs (82.5 nm), while Lactococcus lactis yielded the highest number (3.2 × 10⁹ particles/mL). Lactobacillus plantarum had the highest protein content (0.124 pg/particle), while Lactobacillus salivarius had the greatest lipid content (16.3 µg/particle). Lipid content significantly influenced cytotoxicity in HEK293T cells (r² = 0.366, p = 0.037). Connectivity Map (CMap) analysis revealed correlations between bEVs from Lactobacillus rhamnosus, Lactobacillus fermentum, Lactobacillus acidophilus, and Streptococcus thermophilus and approved drugs for skin health. Experimentally, these bEVs enhanced collagen synthesis in fibroblasts by up to 1.25-fold (p < 0.001). Proteomic analysis identified distinct protein sets for each bEV. Further analysis demonstrated interaction networks between bEV proteins and human proteins that promote collagen production through the JAK-STAT, PI3K-AKT, and focal adhesion pathways. In conclusion, this study highlights the strain-specific characteristics and therapeutic potential of bEVs in promoting collagen production, presenting a novel approach to discovering new indications for bEVs in potential skin care applications.
细菌细胞外囊泡（bEVs）通过模拟微生物群的作用而具有治疗潜力。在此，我们对来自八种革兰氏阳性乳杆菌属菌株的bEVs进行了表征，评估了它们的治疗潜力。在初步表征中，副干酪乳杆菌产生的bEVs最大（82.5纳米），而乳酸乳球菌产生的数量最多（3.2×10⁹颗粒/毫升）。植物乳杆菌的蛋白质含量最高（0.124皮克/颗粒），而唾液乳杆菌的脂质含量最高（16.3微克/颗粒）。脂质含量显著影响HEK293T细胞的细胞毒性（r² = 0.366，p = 0.037）。连接图谱（CMap）分析揭示了来自鼠李糖乳杆菌、发酵乳杆菌、嗜酸乳杆菌和嗜热链球菌的bEVs与已批准的皮肤健康药物之间的相关性。实验表明，这些bEVs可使成纤维细胞中的胶原蛋白合成增加高达1.25倍（p < 0.001）。蛋白质组学分析确定了每种bEV的不同蛋白质组。进一步分析表明，bEV蛋白质与人类蛋白质之间存在相互作用网络，这些网络通过JAK-STAT、PI3K-AKT和粘着斑途径促进胶原蛋白的产生。总之，本研究突出了bEVs在促进胶原蛋白产生方面的菌株特异性特征和治疗潜力，为在潜在皮肤护理应用中发现bEVs的新适应症提供了一种新方法。
