Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Curr Opin Genet Dev. 2023 Aug;81:102067. doi: 10.1016/j.gde.2023.102067. Epub 2023 Jun 23.
Forced expression of a specific set of transcription factors can reprogram terminally differentiated cells and convert them into induced pluripotent stem cells that correspond to cells in the inner cell mass of the developing embryo. It is now recognized that the scope of the reprogramming factors extends far beyond the stem cell biology. Studies using mouse models demonstrated that the induction of the reprogramming factors promotes cellular reprogramming in vivo. Closer inspection of these mice has revealed that expression of the reprogramming factors results in unique consequences that are not seen when cells are reprogrammed ex vivo, and can provide insights into development, tissue regeneration, cancer, and aging.
特定转录因子的强制表达可以重编程终末分化细胞，并将其转化为诱导多能干细胞，这些细胞与发育胚胎的内细胞团中的细胞相对应。现在人们已经认识到，重编程因子的范围远远超出了干细胞生物学。使用小鼠模型的研究表明，重编程因子的诱导促进了体内细胞的重编程。对这些小鼠的更仔细检查表明，重编程因子的表达导致了独特的后果，而这些后果在体外细胞重编程时是不会出现的，并且可以为发育、组织再生、癌症和衰老提供新的见解。
