The Brånemark Clinic, Public Dental Service, Region Västra Götaland, Gothenburg, Sweden.
Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Clin Implant Dent Relat Res. 2024 Apr;26(2):266-280. doi: 10.1111/cid.13241. Epub 2023 Jun 25.
Nonresorbable membranes promote bone formation during guided bone regeneration (GBR), yet the relationships between membrane properties and molecular changes in the surrounding tissue are largely unknown.
To compare the molecular events in the overlying soft tissue, the membrane, and the underlying bone defect during GBR using dual-layered expanded membranes versus dense polytetrafluoroethylene (PTFE) membranes.
Rat femur defects were treated with either dense PTFE (d-PTFE) or dual-layered expanded PTFE (dual e-PTFE) or left untreated as a sham. Samples were collected after 6 and 28 days for gene expression, histology, and histomorphometry analyses.
The two membranes promoted the overall bone formation compared to sham. Defects treated with dual e-PTFE exhibited a significantly higher proportion of new bone in the top central region after 28 days. Compared to that in the sham, the soft tissue in the dual e-PTFE group showed 2-fold higher expression of genes related to regeneration (FGF-2 and FOXO1) and vascularization (VEGF). Furthermore, compared to cells in the d-PTFE group, cells in the dual e-PTFE showed 2.5-fold higher expression of genes related to osteogenic differentiation (BMP-2), regeneration (FGF-2 and COL1A1), and vascularization (VEGF), in parallel with lower expression of proinflammatory cytokines (IL-6 and TNF-α). Multiple correlations were found between the molecular activities in membrane-adherent cells and those in the soft tissue.
Selective surface modification of the two sides of the e-PTFE membrane constitutes a novel means of modulating the tissue response and promoting bone regeneration.
不可吸收膜在引导骨再生（GBR）过程中促进骨形成，但膜的特性与周围组织的分子变化之间的关系在很大程度上尚不清楚。
使用双层扩张膜与致密聚四氟乙烯（PTFE）膜比较 GBR 中覆盖的软组织、膜和下方骨缺损的分子事件。
用致密 PTFE（d-PTFE）或双层扩张 PTFE（双层 e-PTFE）或不做处理作为假手术处理大鼠股骨缺损。在第 6 和 28 天收集样本进行基因表达、组织学和组织形态计量学分析。
与假手术相比，两种膜均促进了整体骨形成。双层 e-PTFE 处理的缺陷在第 28 天的顶部中央区域表现出明显更高比例的新骨。与假手术相比，双层 e-PTFE 组的软组织中与再生（FGF-2 和 FOXO1）和血管生成（VEGF）相关的基因表达增加了 2 倍。此外，与 d-PTFE 组的细胞相比，双层 e-PTFE 组的细胞中与成骨分化（BMP-2）、再生（FGF-2 和 COL1A1）和血管生成（VEGF）相关的基因表达增加了 2.5 倍，同时炎症细胞因子（IL-6 和 TNF-α）的表达降低。在膜附着细胞和软组织中的分子活性之间发现了多个相关性。
e-PTFE 膜两侧的选择性表面修饰构成了调节组织反应和促进骨再生的新方法。
