Univ Angers, INSERM, CNRS, MITOVASC, Team 2 CarMe, SFR ICAT, 49000 Angers, France.
Laboratory of Applied Biotechnology (LBA3B), Azm Center for Research in Biotechnology and Its Applications, EDST, Lebanese University, Tripoli 1300, Lebanon.
Infect Disord Drug Targets. 2024;24(1):e230623218222. doi: 10.2174/1871526523666230623152045.
Bee venoms are well-known for their important biological activities. More specifically, the venom of Apis mellifera syriaca was shown to exhibit various biological effects, including antimicrobial effects. It is suggested that the anti-microbial effect of venom could be accompanied by an immunomodulatory response in the host favoring anti-inflammatory responses. Thus, in this work, we investigated, for the first time, the immunomodulatory effects of venom in mice. Firstly, it was found that this venom exhibited mild toxicity in BALB/c mice after intraperitoneal injection with an LD50 of 3.8 mg/kg. We then investigated its immunomodulatory effects by evaluating the splenic levels of both pro- and anti-inflammatory cytokines in mice by ELISA. Interestingly, at 1 mg/kg, venom induced a decrease in IFN-γ, TNF-α, IL-4, and IL-10 at 24h postinjection. At a higher dose (3 mg/kg), an increase in IFN-γ and IL-4 levels was observed, while the levels of TNF-α and IL-10 remained low compared to the control. Altogether, these preliminary data suggest that venom exhibits anti-inflammatory effects at a sublethal dose (1 mg/kg), while at a higher dose (3 mg/kg), it induces inflammatory effects.
蜂毒液以其重要的生物学活性而闻名。更具体地说，研究表明，阿萨伊蜜蜂的毒液具有多种生物学效应，包括抗菌作用。有人认为，毒液的抗菌作用可能伴随着宿主的免疫调节反应，有利于抗炎反应。因此，在这项工作中，我们首次研究了毒液对小鼠的免疫调节作用。首先，发现该毒液在腹腔注射后对 BALB/c 小鼠表现出轻微的毒性，LD50 为 3.8mg/kg。然后，我们通过 ELISA 评估了小鼠脾脏中促炎和抗炎细胞因子的水平来研究其免疫调节作用。有趣的是，在 1mg/kg 剂量下，毒液在注射后 24 小时诱导 IFN-γ、TNF-α、IL-4 和 IL-10 水平降低。在较高剂量（3mg/kg）下，观察到 IFN-γ和 IL-4 水平升高，而 TNF-α和 IL-10 水平与对照组相比仍较低。总的来说，这些初步数据表明，毒液在亚致死剂量（1mg/kg）下表现出抗炎作用，而在较高剂量（3mg/kg）下，它诱导炎症反应。
