Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Ankara University, Ankara, Turkey.
Muğla Vocational School Department of Chemistry and Chemical Treatment Technologies, MuğlaSıtkıKoçman University, Muğla, Turkey.
Appl Biochem Biotechnol. 2023 May;195(5):3194-3205. doi: 10.1007/s12010-022-04284-x. Epub 2022 Dec 27.
Extraction and characterization of natural products provide the opportunity to expand our arsenal of drug candidates against a wide range of diseases including cancer and inflammatory disorders. Previous studies have shown bee venom to have immense potential as an anti-inflammatory drug candidate. In this study, we focused on the venom of Apis mellifera anatoliaca and characterized its content by HPLC. An in vitro inflammation model based on lipopolysaccharide (LPS)-stimulated mammalian macrophages was utilized to examine the venom's anti-inflammatory potential. Additionally, its antiproliferative activity was evaluated in vitro against a human glioblastoma cell line. Based on the TNF, IL6, GMCSF, and IL12p40 pro-inflammatory cytokine production level in LPS-induced macrophages, venom-treated groups showed substantial decrease in the inflammatory action compared to untreated LPS-stimulated macrophages. When the cells were analyzed for viability, the venom did not have any cytotoxic effect on the macrophages at the concentration ranges that were utilized. Moreover, IC value of the venom was above 60 µg/mL on glioblastoma cancer cell line. These results suggest that the Apis mellifera anatoliaca venom does not have anticancer drug candidate potential, whereas it can efficiently be used against inflammatory and autoimmune disorders. To our knowledge, this is the first study to specifically examine the effect of anti-inflammatory activity of Apis mellifera anatoliaca venom on macrophages.
天然产物的提取和特性研究为我们提供了机会，使我们能够扩大候选药物的种类，以对抗包括癌症和炎症性疾病在内的广泛疾病。先前的研究表明，蜂毒具有作为抗炎候选药物的巨大潜力。在这项研究中，我们专注于研究 Apis mellifera anatoliaca 的毒液，并通过 HPLC 对其成分进行了表征。我们利用基于脂多糖（LPS）刺激的哺乳动物巨噬细胞的体外炎症模型来研究毒液的抗炎潜力。此外，还在体外评估了其对人神经胶质瘤细胞系的抗增殖活性。基于 LPS 诱导的巨噬细胞中 TNF、IL6、GMCSF 和 IL12p40 促炎细胞因子的产生水平，与未经处理的 LPS 刺激的巨噬细胞相比，毒液处理组的炎症作用明显降低。当对细胞进行活力分析时，在使用的浓度范围内，毒液对巨噬细胞没有任何细胞毒性作用。此外，毒液对神经胶质瘤癌细胞系的 IC 值大于 60µg/mL。这些结果表明，Apis mellifera anatoliaca 毒液没有抗癌候选药物的潜力，而它可以有效地用于治疗炎症和自身免疫性疾病。据我们所知，这是第一项专门研究 Apis mellifera anatoliaca 毒液对巨噬细胞抗炎活性的影响的研究。
