用于检测核酸和非核酸靶标的CRISPR/Cas系统。
CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets.
作者信息
Su Weiran, Li Junru, Ji Chen, Chen Congshuo, Wang Yuzheng, Dai Huili, Li Fengqin, Liu Peifeng
机构信息
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200032 China.
Central Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127 China.
出版信息
Nano Res. 2023 Mar 20:1-14. doi: 10.1007/s12274-023-5567-4.
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are becoming powerful tools for disease biomarkers detection. Due to the specific recognition, -cleavage and nonspecific -cleavage capabilities, CRISPR/Cas systems have implemented the detection of nucleic acid targets (DNA and RNA) as well as non-nucleic acid targets (e.g., proteins, exosomes, cells, and small molecules). In this review, we first summarize the principles and characteristics of various CRISPR/Cas systems, including CRISPR/Cas9, Cas12, Cas13 and Cas14 systems. Then, various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically. Finally, the prospects and challenges of their applications in biosensing are discussed.
成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）系统正成为疾病生物标志物检测的强大工具。由于具有特异性识别、切割和非特异性切割能力，CRISPR/Cas系统已实现对核酸靶标（DNA和RNA）以及非核酸靶标（如蛋白质、外泌体、细胞和小分子）的检测。在本综述中，我们首先总结了各种CRISPR/Cas系统的原理和特性，包括CRISPR/Cas9、Cas12、Cas13和Cas14系统。然后，着重介绍了CRISPR/Cas系统在检测核酸和非核酸靶标方面的各类应用。最后，讨论了它们在生物传感应用中的前景和挑战。
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