Koruda M J, Zimbler A, Settle R G, Jacobs D O, Rolandelli R H, Wolf G L, Rombeau J L
J Surg Res. 1986 May;40(5):475-81. doi: 10.1016/0022-4804(86)90218-0.
There is no accurate noninvasive method for differentiating between partial-thickness and full-thickness cutaneous burn wounds. Full-thickness burns (FTB) result in slower resorption of wound edema than partial thickness burns (PTB). Since proton NMR parameters, particularly the T1 relaxation time, correlate with tissue water content (TWC), the present study determined whether proton NMR could distinguish PTB from FTB. An area of upper dorsum (approximately 15% BSA) of 35 adult rats was immersed in boiling water for either 3 sec (PTB) or 10 sec (FTB). In 10 control rats, the same area was immersed in room temperature water. Rats were sacrificed at either 3 or 48 hr after burn, and skin samples were analyzed to determine spin-lattice (T1) and spin-spin (T2) relaxation times. TWC was then measured gravimetrically by desiccation. Both T1 and T2 times significantly correlated with TWC (T1: r = 0.74, P less than 0.0001; T2: r = 0.75; P less than 0.0001). Both PTB and FTB resulted in significant elevations of T1, T2, and TWC 3 hr after injury (P less than 0.001). At 48 hr postburn the T1, T2, and TWC of the PTB group had decreased to control values (P less than 0.05), while all FTB parameters remained significantly elevated as compared to both the control and the 48-hr PTB parameters (P less than 0.001). In vitro NMR measurements distinguish PTB from FTB in this rat model within 48 hr. These data provide a basis for investigating in vitro NMR techniques for the noninvasive assessment of burn wound depth.
目前尚无准确的非侵入性方法来区分部分厚度和全层皮肤烧伤创面。全层烧伤（FTB）导致创面水肿的吸收比部分厚度烧伤（PTB）更慢。由于质子核磁共振参数，特别是T1弛豫时间，与组织含水量（TWC）相关，因此本研究确定质子核磁共振是否能够区分PTB和FTB。将35只成年大鼠的上背部区域（约占体表面积的15%）浸入沸水中3秒（PTB）或10秒（FTB）。在10只对照大鼠中，将相同区域浸入室温水中。在烧伤后3小时或48小时处死大鼠，并分析皮肤样本以确定自旋晶格（T1）和自旋自旋（T2）弛豫时间。然后通过干燥重量法测量TWC。T1和T2时间均与TWC显著相关（T1：r = 0.74，P < 0.0001；T2：r = 0.75；P < 0.0001）。PTB和FTB在损伤后3小时均导致T1、T2和TWC显著升高（P < 0.001）。烧伤后48小时，PTB组的T1、T2和TWC已降至对照值（P < 0.05），而与对照组和48小时PTB组参数相比，所有FTB参数仍显著升高（P < 0.001）。在该大鼠模型中，体外核磁共振测量在48小时内可区分PTB和FTB。这些数据为研究用于烧伤创面深度无创评估的体外核磁共振技术提供了依据。
