Tewari Priyamvada, Garritano James, Bajwa Neha, Sung Shijun, Huang Haochong, Wang Dayong, Grundfest Warren, Ennis Daniel B, Ruan Dan, Brown Elliott, Dutson Erik, Fishbein Michael C, Taylor Zachary
Department of Bioengineering, University of Los Angeles, California, 410 Westwood Plaza, Los Angeles, CA 90025, USA.
Department of Electrical Engineering, University of Los Angeles, California, 410 Westwood Plaza, Los Angeles, CA 90025, USA.
Biomed Opt Express. 2018 Dec 21;10(1):322-337. doi: 10.1364/BOE.10.000322. eCollection 2019 Jan 1.
A method to register THz and visible images of cutaneous burn wounds and to calibrate THz image data is presented. Images of partial and full thickness burn wounds in 9 rats were collected over 435 mins. = 7.25 hours following burn induction. A two-step process was developed to reference the unknown structure of THz imaging contrast to the known structure and the features present in visible images of the injury. This process enabled the demarcation of a wound center for each THz image, independent of THz contrast. Threshold based segmentation enabled the automated identification of air (0% reflectivity), brass (100% reflectivity), and abdomen regions within the registered THz images. Pixel populations, defined by the segmentations, informed unsupervised image calibration and contrast warping for display. The registered images revealed that the largest variation in THz tissue reflectivity occurred superior to the contact region at ~0.13%/min. Conversely the contact region showed demonstrated an ~6.5-fold decrease at ~0.02%/min. Exploration of occlusion effects suggests that window contact may affect the measured edematous response.
本文提出了一种用于配准皮肤烧伤创面的太赫兹（THz）图像和可见光图像以及校准太赫兹图像数据的方法。在9只大鼠身上诱导烧伤后435分钟（即7.25小时）内收集了部分厚度和全层厚度烧伤创面的图像。开发了一个两步过程，将太赫兹成像对比度的未知结构与已知结构以及损伤可见光图像中存在的特征进行关联。该过程能够为每张太赫兹图像划定伤口中心，而与太赫兹对比度无关。基于阈值的分割能够自动识别配准后的太赫兹图像中的空气（0%反射率）、黄铜（100%反射率）和腹部区域。由分割定义的像素群体为无监督图像校准和用于显示的对比度扭曲提供了依据。配准后的图像显示，太赫兹组织反射率的最大变化发生在接触区域上方，约为0.13%/分钟。相反，接触区域显示出以约0.02%/分钟的速度下降约6.5倍。对闭塞效应的研究表明，窗口接触可能会影响测得的水肿反应。
