• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

抗菌肽cathelicidin-BF在体内的高表达及其活性验证。

High expression of antimicrobial peptides cathelicidin-BF in and verification of its activity.

作者信息

Dong Xufeng, Shan Hu, Wang Shubai, Jiang Zhengjun, Wang Shaojuan, Qin Zhihua

机构信息

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, Shandong, China.

College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China.

出版信息

Front Microbiol. 2023 Jun 9;14:1153365. doi: 10.3389/fmicb.2023.1153365. eCollection 2023.

DOI:10.3389/fmicb.2023.1153365
PMID:37362941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10288212/
Abstract

Antibacterial peptides are endogenous polypeptides produced by multicellular organisms to protect the host against pathogenic microbes, they show broad spectrum antimicrobial activities against various microorganisms and possess low propensity for developing resistance. The purpose of this study is to develop recombinant antibacterial peptide cathelicidin-BF by genetic engineering and protein engineering technology, and study its antibacterial activity and , so as to provide reference for the production and application of recombinant antibacterial peptide cathelicidin-BF. In this study, on account of eukaryotic expression system, we expressed and prepared antibacterial peptide cathelicidin-BF. Then, the minimum inhibitory concentration of antibacterial peptide cathelicidin-BF and the comparison with the antibacterial activity of antibiotics were determined through the antibacterial experiment . Chickens as infection model were used to verify the antibacterial peptide activity . The results show that the bacteriostatic ability of antibacterial peptide cathelicidin-BF is similar to that of antibiotics in certain concentration, and can reach the treatment level of antibiotics. Although the mode of administration of antibacterial peptide is still limited, this study can provide reference for the future research of antibacterial peptide.

摘要

抗菌肽是多细胞生物产生的内源性多肽，用于保护宿主抵御病原微生物，它们对各种微生物具有广谱抗菌活性，且产生耐药性的倾向较低。本研究的目的是通过基因工程和蛋白质工程技术开发重组抗菌肽cathelicidin-BF，并研究其抗菌活性，为重组抗菌肽cathelicidin-BF的生产和应用提供参考。在本研究中，基于真核表达系统，我们表达并制备了抗菌肽cathelicidin-BF。然后，通过抗菌实验确定了抗菌肽cathelicidin-BF的最低抑菌浓度以及与抗生素抗菌活性的比较。以鸡作为感染模型来验证抗菌肽的活性。结果表明，抗菌肽cathelicidin-BF在一定浓度下的抑菌能力与抗生素相似，且能达到抗生素的治疗水平。虽然抗菌肽的给药方式仍有限，但本研究可为抗菌肽的未来研究提供参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/c93dab19cc42/fmicb-14-1153365-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/cb4990c3235b/fmicb-14-1153365-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/83dfd550606b/fmicb-14-1153365-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/09ec71c781cf/fmicb-14-1153365-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/7fd5aa060986/fmicb-14-1153365-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/52ea02cd597e/fmicb-14-1153365-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/a63b9b5949d9/fmicb-14-1153365-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/c93dab19cc42/fmicb-14-1153365-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/cb4990c3235b/fmicb-14-1153365-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/83dfd550606b/fmicb-14-1153365-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/09ec71c781cf/fmicb-14-1153365-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/7fd5aa060986/fmicb-14-1153365-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/52ea02cd597e/fmicb-14-1153365-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/a63b9b5949d9/fmicb-14-1153365-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79d0/10288212/c93dab19cc42/fmicb-14-1153365-g007.jpg

相似文献

1
High expression of antimicrobial peptides cathelicidin-BF in and verification of its activity.抗菌肽cathelicidin-BF在体内的高表达及其活性验证。
Front Microbiol. 2023 Jun 9;14:1153365. doi: 10.3389/fmicb.2023.1153365. eCollection 2023.
2
Snake cathelicidin from Bungarus fasciatus is a potent peptide antibiotics.来自银环蛇的蛇抗菌肽是一种强效肽类抗生素。
PLoS One. 2008 Sep 16;3(9):e3217. doi: 10.1371/journal.pone.0003217.
3
Cathelicidin-BF, a snake cathelicidin-derived antimicrobial peptide, could be an excellent therapeutic agent for acne vulgaris.抗菌肽 BF 是一种来源于蛇的抗菌肽，对治疗寻常痤疮可能是一种极好的治疗药物。
PLoS One. 2011;6(7):e22120. doi: 10.1371/journal.pone.0022120. Epub 2011 Jul 15.
4
The Expression of Antibacterial Peptide Turgencin A in and an Analysis of Its Antibacterial Activity.在 中表达抗菌肽 Turgencin A 及其抗菌活性分析。
Molecules. 2023 Jul 14;28(14):5405. doi: 10.3390/molecules28145405.
5
Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in .抗菌肽fowlicidin-2在……中的重组表达及生物学特性研究 （原文句子不完整，推测补充完整后的翻译）
Exp Ther Med. 2016 Oct;12(4):2324-2330. doi: 10.3892/etm.2016.3578. Epub 2016 Aug 5.
6
Antibacterial peptide PMAP-37(F34-R), expressed in Pichia pastoris, is effective against pathogenic bacteria and preserves plums.毕赤酵母表达的抗菌肽 PMAP-37(F34-R)可有效对抗病原菌并能保鲜李果。
Microb Cell Fact. 2023 Aug 27;22(1):164. doi: 10.1186/s12934-023-02164-5.
7
The antibacterial activity of BF-30 in vitro and in infected burned rats is through interference with cytoplasmic membrane integrity.BF-30 的体外抗菌活性和感染烧伤大鼠的体内抗菌活性是通过干扰细胞质膜完整性来实现的。
Peptides. 2011 Jun;32(6):1131-8. doi: 10.1016/j.peptides.2011.04.002. Epub 2011 Apr 14.
8
Efficient production of a recombinant Venerupis philippinarum defensin (VpDef) in Pichia pastoris and characterization of its antibacterial activity and stability.在毕赤酵母中高效生产重组菲律宾蛤仔防御素（VpDef）及其抗菌活性和稳定性的表征。
Protein Expr Purif. 2018 Jul;147:78-84. doi: 10.1016/j.pep.2018.03.001. Epub 2018 Mar 7.
9
Short, Synthetic Cationic Peptides Have Antibacterial Activity against Mycobacterium smegmatis by Forming Pores in Membrane and Synergizing with Antibiotics.短而合成的阳离子肽通过在膜中形成孔并与抗生素协同作用对耻垢分枝杆菌具有抗菌活性。
Antibiotics (Basel). 2015 Aug 24;4(3):358-78. doi: 10.3390/antibiotics4030358.
10
The antimicrobial peptide cathelicidin-BF could be a potential therapeutic for Salmonella typhimurium infection.抗菌肽 cathelicidin-BF 可能成为治疗鼠伤寒沙门氏菌感染的一种潜在疗法。
Microbiol Res. 2015 Feb;171:45-51. doi: 10.1016/j.micres.2014.12.009. Epub 2015 Jan 3.

引用本文的文献

1
Influence of antibacterial peptides in drinking water on growth and intestinal health of European meat pigeon.饮用水中抗菌肽对欧洲肉鸽生长性能及肠道健康的影响
Poult Sci. 2025 Aug 6;104(10):105636. doi: 10.1016/j.psj.2025.105636.
2
Porcine β-Defensin 2 Expressed in Alleviates Enterotoxigenic -Induced Intestinal Injury and Inflammatory Response in Mice.在小鼠中表达的猪β-防御素2减轻产肠毒素大肠杆菌诱导的肠道损伤和炎症反应。
Animals (Basel). 2025 May 11;15(10):1389. doi: 10.3390/ani15101389.
3
Expression of recombination antimicrobial protein PIL22-PBD-2 in Pichia pastoris and verification of its biological function in vitro.

本文引用的文献

1
Optimized Expression and Isolation of Recombinant Active Secreted Proteases Using .使用……优化重组活性分泌蛋白酶的表达与分离
Bio Protoc. 2023 Mar 5;13(5):e4628. doi: 10.21769/BioProtoc.4628.
2
Challenges and progress towards industrial recombinant protein production in yeasts: A review.酵母中工业重组蛋白生产的挑战与进展：综述。
Biotechnol Adv. 2023 May-Jun;64:108121. doi: 10.1016/j.biotechadv.2023.108121. Epub 2023 Feb 10.
3
Evaluation of the efficacy of the antimicrobial peptide HJH-3 in chickens infected with Pullorum.
重组抗菌蛋白PIL22-PBD-2在毕赤酵母中的表达及其体外生物学功能验证
Vet Res. 2025 Mar 7;56(1):52. doi: 10.1186/s13567-024-01428-1.
4
Cathelicidins: Opportunities and Challenges in Skin Therapeutics and Clinical Translation.Cathelicidins：皮肤治疗与临床转化中的机遇与挑战
Antibiotics (Basel). 2024 Dec 24;14(1):1. doi: 10.3390/antibiotics14010001.
5
Antimicrobial Peptides: Mechanism, Expressions, and Optimization Strategies.抗菌肽：作用机制、表达及优化策略
Probiotics Antimicrob Proteins. 2025 Apr;17(2):857-872. doi: 10.1007/s12602-024-10391-4. Epub 2024 Nov 11.
6
Antimicrobial peptide-based strategies to overcome antimicrobial resistance.基于抗菌肽的策略来克服抗菌耐药性。
Arch Microbiol. 2024 Sep 23;206(10):411. doi: 10.1007/s00203-024-04133-x.
抗菌肽HJH-3对感染鸡白痢的鸡的疗效评估。
Front Microbiol. 2023 Jan 24;14:1102789. doi: 10.3389/fmicb.2023.1102789. eCollection 2023.
4
Oral antimicrobial peptide-EGCG nanomedicines for synergistic treatment of ulcerative colitis.口服抗菌肽-EGCG 纳米药物协同治疗溃疡性结肠炎。
J Control Release. 2022 Jul;347:544-560. doi: 10.1016/j.jconrel.2022.05.025. Epub 2022 May 25.
5
Effects of antibacterial peptide combinations on growth performance, intestinal health, and immune function of broiler chickens.抗菌肽组合对肉鸡生长性能、肠道健康和免疫功能的影响。
Poult Sci. 2020 Dec;99(12):6481-6492. doi: 10.1016/j.psj.2020.08.068. Epub 2020 Sep 14.
6
Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins.毕赤酵母：一种高效的表达系统，可用于最优合成异源蛋白。
J Cell Physiol. 2020 Sep;235(9):5867-5881. doi: 10.1002/jcp.29583. Epub 2020 Feb 14.
7
Therapeutic administration of the recombinant antimicrobial peptide microcin J25 effectively enhances host defenses against gut inflammation and epithelial barrier injury induced by enterotoxigenic Escherichia coli infection.重组抗菌肽微菌素 J25 的治疗给药可有效增强宿主对肠毒素性大肠杆菌感染引起的肠道炎症和上皮屏障损伤的防御能力。
FASEB J. 2020 Jan;34(1):1018-1037. doi: 10.1096/fj.201901717R. Epub 2019 Nov 28.
8
Oral administration of transgenic biosafe microorganism containing antimicrobial peptide enhances the survival of tilapia fry infected bacterial pathogen.口服含有抗菌肽的转基因生物安全微生物可提高感染细菌病原体的罗非鱼鱼苗的存活率。
Fish Shellfish Immunol. 2019 Dec;95:606-616. doi: 10.1016/j.fsi.2019.10.052. Epub 2019 Nov 1.
9
Continuous Subcutaneous Delivery of Proline-Rich Antimicrobial Peptide Api137 Provides Superior Efficacy to Intravenous Administration in a Mouse Infection Model.在小鼠感染模型中，富含脯氨酸的抗菌肽Api137的持续皮下给药比静脉给药具有更高的疗效。
Front Microbiol. 2019 Oct 2;10:2283. doi: 10.3389/fmicb.2019.02283. eCollection 2019.
10
DRAMP 2.0, an updated data repository of antimicrobial peptides.DRAMP 2.0，一个更新的抗菌肽数据库。
Sci Data. 2019 Aug 13;6(1):148. doi: 10.1038/s41597-019-0154-y.