Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control / National Coarse Cereals Engineering Research Center / Key Laboratory of Low-Carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs P. R. China, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China.
Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control / National Coarse Cereals Engineering Research Center / Key Laboratory of Low-Carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs P. R. China, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China.
Gene. 2023 Aug 30;879:147593. doi: 10.1016/j.gene.2023.147593. Epub 2023 Jun 24.
Plant glutamate receptor proteins (GLRs) are involved in plant development, biotic stress, and light-signal transduction. Vigna angularis is a traditional crop with important economic value in China, and the identification of functional genes can facilitate the breeding of stress resistant varieties. Here, we identified the members of the GLR gene family in the adzuki bean genome and investigated gene expression under light and rust fungal (Uromyces vignae) stimuli. Sixteen GLR genes were identified in V. angularis (VaGLRs), and these genes clustered in a single clade (clade III) with two groups. Evolutionary analysis showed that three VaGLRs result from tandem duplications and four result from whole genome/segmental duplications. To understand the regulation of expression of VaGLRs, cis-acting elements were analyzed in the promoter regions of the VaGLRs including cis-acting elements associated with light and stress responsiveness. Expression analysis by qRT-PCR revealed transcripts of eight and 10 VaGLRs in response to light stimuli and rust infection, respectively. For light responsiveness, the expression levels of XP_017430569.1 and XP_017425299.1 were higher under light condition than in darkness, while the expression levels of XP_017406996.1, XP_017425763.1, and XP_017423557.1 gradually recovered during dark treatment. Additionally, the relative expression levels of XP_017413816.1, XP_017436268.1, and XP_017425299.1 were significantly elevated during U. vignae infection in a resistant cultivar compared to the expression levels in a susceptible cultivar. XP_017425299.1 expression was induced both by light and rust infection, suggesting this gene may link light and disease resistance signaling pathways. Our results provide insight into how the VaGLRs contribute to adzuki bean response to light stimulus and pathogen attack. These identified VaGLRs also provide important reference to improve adzuki bean germplasm resources.
植物谷氨酸受体蛋白（GLRs）参与植物发育、生物胁迫和光信号转导。豇豆是中国一种具有重要经济价值的传统作物，鉴定功能基因可以促进抗逆品种的选育。在这里，我们鉴定了菜豆基因组中 GLR 基因家族的成员，并研究了光照和锈菌（Uromyces vignae）刺激下的基因表达。在 V. angularis 中鉴定出 16 个 GLR 基因（VaGLRs），这些基因聚集在一个单一的分支（分支 III）中，分为两组。进化分析表明，三个 VaGLR 是串联重复的结果，四个是全基因组/片段重复的结果。为了了解 VaGLR 表达的调控，分析了 VaGLR 启动子区的顺式作用元件，包括与光和应激反应相关的顺式作用元件。qRT-PCR 表达分析显示，8 个和 10 个 VaGLR 在光照刺激和锈菌感染下分别有转录本。对于光响应，XP_017430569.1 和 XP_017425299.1 的表达水平在光照条件下高于黑暗条件下，而 XP_017406996.1、XP_017425763.1 和 XP_017423557.1 的表达水平在黑暗处理过程中逐渐恢复。此外，在抗病品种中，相对表达水平 XP_017413816.1、XP_017436268.1 和 XP_017425299.1 在 U. vignae 感染期间明显高于感病品种。XP_017425299.1 的表达既受光诱导，也受锈菌感染诱导，表明该基因可能连接光和抗病信号通路。我们的研究结果提供了有关 VaGLR 如何促进菜豆对光刺激和病原体攻击的反应的见解。这些鉴定出的 VaGLR 也为提高菜豆种质资源提供了重要参考。
