Yin Zhengong, Meng Xianxin, Guo Yifan, Wei Shuhong, Lai Yongcai, Wang Qiang
Crop Resources Institute of Heilongjiang Academy of Agricultural Sciences Harbin, Heilongjiang, China.
Front Genet. 2022 Apr 25;13:847612. doi: 10.3389/fgene.2022.847612. eCollection 2022.
Adzuki bean () is an important dietary legume crop that was first cultivated and domesticated in Asia. Currently, little is known concerning the evolution and expression patterns of the basic leucine zipper (bZIP) family transcription factors in the adzuki bean. Through the PFAM search, 72 bZIP members of adzuki bean (VabZIP) were identified from the reference genome. Most of them were located on 11 chromosomes and seven on an unknown chromosome. A comprehensive analysis, including evolutionary, motifs, gene structure, -elements, and collinearity was performed to identify VabZIP members. The subcellular localization results showed VabZIPs might locate on the nuclear. Quantitative real-time PCR (qRT-PCR) analysis of the relative expression of VabZIPs in different tissues at the bud stage revealed that VabZIPs had a tissue-specific expression pattern, and its expression was influenced by abiotic stress. These characteristics of provide insights for future research aimed at developing interventions to improve abiotic stress resistance
小豆(Vigna angularis)是一种重要的食用豆类作物,最早在亚洲被种植和驯化。目前,关于小豆中碱性亮氨酸拉链(bZIP)家族转录因子的进化和表达模式知之甚少。通过PFAM搜索,从小豆参考基因组中鉴定出72个bZIP成员(VabZIP)。其中大部分位于11条染色体上,7个位于一条未知染色体上。对VabZIP成员进行了包括进化、基序、基因结构、顺式作用元件和共线性在内的综合分析。亚细胞定位结果表明VabZIPs可能定位于细胞核。对芽期不同组织中VabZIPs相对表达量的实时荧光定量PCR(qRT-PCR)分析表明,VabZIPs具有组织特异性表达模式,其表达受非生物胁迫影响。这些特性为今后旨在开发提高非生物胁迫抗性干预措施的研究提供了思路 。