School of Biosciences and Technology, Vellore Institute of Technology, Vellore, India.
Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology, Vellore, India.
Biotechnol Bioeng. 2023 Aug;120(8):2117-2132. doi: 10.1002/bit.28481. Epub 2023 Jun 27.
Among several types of brain cancers, glioblastoma multiforme (GBM) is a terminal and aggressive disease with a median survival of 15 months despite the most intensive surgery and chemotherapy. Preclinical models that accurately reproduce the tumor microenvironment are vital for developing new therapeutic alternatives. Understanding the complicated interactions between cells and their surroundings is essential to comprehend the tumor's microenvironment, however the monolayer cell culture approach falls short. Numerous approaches are used to develop GBM cells into tumor spheroids, while scaffold-based spheroids provides the opportunity to investigate the synergies between cells as well as cells and the matrix. This review summarizes the development of various scaffold-based GBM spheroid models and the prospective for their use as drug testing systems.
在几种类型的脑癌中，多形性胶质母细胞瘤（GBM）是一种终末期和侵袭性疾病，尽管进行了最积极的手术和化疗，中位生存期仍为 15 个月。能够准确再现肿瘤微环境的临床前模型对于开发新的治疗选择至关重要。了解细胞与其周围环境之间的复杂相互作用对于理解肿瘤的微环境至关重要，但是单层细胞培养方法存在不足。目前有许多方法可用于将 GBM 细胞培养成肿瘤球体，而基于支架的球体则为研究细胞之间以及细胞与基质之间的协同作用提供了机会。本综述总结了各种基于支架的 GBM 球体模型的发展，并展望了它们作为药物测试系统的应用前景。
