Ishibashi Yu, Haraguchi Ryota, Aoki Shigehisa, Oishi Yushi, Narita Takayuki
Department of Chemistry and Applied Chemistry, Saga University, Saga 840-8502, Japan.
Department of Pathology and Microbiology, Saga University, Saga 849-8501, Japan.
Gels. 2023 Jun 2;9(6):458. doi: 10.3390/gels9060458.
Hollow collagen gels are promising materials for drug/cell delivery systems to promote tissue regeneration because they may be able to function as carriers for these types of loads. Controlling the cavity size and swelling suppression is essential to expand the applications and improve the usability of such gel-like systems. We investigated the effects of UV-treated collagen solutions as a pre-gel aqueous mixture on the formation and properties of the hollow collagen gels in terms of their preparation range limits, morphology, and swelling ratio. The UV treatment thickened the pre-gel solutions, which allowed hollowing at lower collagen concentrations. This treatment also prevents the over-swelling of the hollow collagen rods in PBS buffer solutions. The UV-treated collagen solutions provided a large lumen space in the prepared collagen hollow fiber rods with a limited swelling ratio, allowing vascular endothelial cells and ectodermal cells to be cultured separately in the outer and inner lumen.
中空胶原凝胶是用于药物/细胞递送系统以促进组织再生的有前景的材料，因为它们可能能够作为这些类型负载的载体。控制腔体大小和抑制膨胀对于扩展此类凝胶状系统的应用和提高其可用性至关重要。我们研究了紫外线处理的胶原溶液作为预凝胶水性混合物对中空胶原凝胶的形成和性质的影响，包括其制备范围限制、形态和膨胀率。紫外线处理使预凝胶溶液变稠，这使得在较低胶原浓度下能够形成中空结构。这种处理还可防止中空胶原棒在PBS缓冲溶液中过度膨胀。紫外线处理的胶原溶液在制备的胶原中空纤维棒中提供了较大的管腔空间，且膨胀率有限，使得血管内皮细胞和外胚层细胞能够分别在内腔和外腔中培养。
