González-Lozano Katia Jamileth, Aréchiga-Carvajal Elva Teresa, Jiménez-Salas Zacarías, Valdez-Rodríguez Debany Marlen, León-Ramírez Claudia Geraldine, Ruiz-Herrera José, Adame-Rodríguez Juan Manuel, López-Cabanillas-Lomelí Manuel, Campos-Góngora Eduardo
Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología, LMYF, Unidad de Manipulación Genética, Monterrey CP 66455, Nuevo León, Mexico.
Universidad Autónoma de Nuevo León, Centro de Investigación en Nutrición y Salud Pública, Monterrey CP 64460, Nuevo León, Mexico.
J Fungi (Basel). 2023 May 23;9(6):600. doi: 10.3390/jof9060600.
is a dimorphic fungus used as a model organism to investigate diverse biotechnological and biological processes, such as cell differentiation, heterologous protein production, and bioremediation strategies. However, little is known about the biological processes responsible for cation concentration homeostasis. Metals play pivotal roles in critical biochemical processes, and some are toxic at unbalanced intracellular concentrations. Membrane transport proteins control intracellular cation concentrations. Analysis of the genome revealed a characteristic functional domain of the cation efflux protein family, i.e., YALI0F19734g, which encodes YALI0F19734p (a putative -Dmct protein), which is related to divalent metal cation tolerance. We report the in silico analysis of the putative -Dmct protein's characteristics and the phenotypic response to divalent cations (Ca, Cu, Fe, and Zn) in the presence of mutant strains, Δ and R, constructed by deletion and reinsertion of the gene, respectively. The absence of the -Dmct protein induces cellular and growth rate changes, as well as dimorphism differences, when calcium, copper, iron, and zinc are added to the cultured medium. Interestingly, the parental and mutant strains were able to internalize the ions. Our results suggest that the protein encoded by the gene is involved in cell development and cation homeostasis in .
是一种二态真菌，用作模式生物来研究各种生物技术和生物学过程，如细胞分化、异源蛋白生产和生物修复策略。然而，对于负责阳离子浓度稳态的生物学过程知之甚少。金属在关键的生化过程中起关键作用，有些在细胞内浓度不平衡时具有毒性。膜转运蛋白控制细胞内阳离子浓度。对基因组的分析揭示了阳离子外流蛋白家族的一个特征性功能域，即YALI0F19734g，它编码YALI0F19734p（一种假定的-Dmct蛋白），与二价金属阳离子耐受性有关。我们报告了对假定的-Dmct蛋白特征的计算机分析，以及在分别通过缺失和重新插入该基因构建的突变菌株Δ和R存在的情况下，对二价阳离子（钙、铜、铁和锌）的表型反应。当向培养基中添加钙、铜、铁和锌时，-Dmct蛋白的缺失会诱导细胞和生长速率变化以及二态性差异。有趣的是，亲本菌株和突变菌株都能够内化这些离子。我们的结果表明，该基因编码的蛋白参与了细胞发育和阳离子稳态。
