Wu Di, Zhuang Fengchao, Wang Jiarui, Gao Ruiqi, Zhang Qiunan, Wang Xiao, Zhang Guochao, Fang Minghui, Zhang Yang, Li Yuhua, Guan Le, Gao Yanqiang
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
College of Life Science, Northeast Forestry University, Harbin 150040, China.
Metabolites. 2023 Jun 10;13(6):742. doi: 10.3390/metabo13060742.
Flower color is an important characteristic of ornamental plants and is determined by various chemical components, including anthocyanin. In the present study, combined metabolomics and transcriptomics analysis was used to explore color variations in the chrysanthemums of three cultivars, of which the color of JIN is yellow, FEN is pink, and ZSH is red. A total of 29 different metabolites, including nine anthocyanins, were identified in common in the three cultivars. Compared with the light-colored cultivars, all of the nine anthocyanin contents were found to be up-regulated in the dark-colored ones. The different contents of pelargonidin, cyanidin, and their derivates were found to be the main reason for color variations. Transcriptomic analysis showed that the color difference was closely related to anthocyanin biosynthesis. The expression level of anthocyanin structural genes, including , , , , and , was in accordance with the flower color depth. This finding suggests that anthocyanins may be a key factor in color variations among the studied cultivars. On this basis, two special metabolites were selected as biomarkers to assist in chrysanthemum breeding for color selection.
花色是观赏植物的一个重要特征，由包括花青素在内的多种化学成分决定。在本研究中，采用代谢组学和转录组学联合分析方法，探究了三个菊花品种的颜色变异情况，其中‘金’为黄色，‘粉’为粉色，‘朱砂红’为红色。在这三个品种中总共鉴定出29种不同的代谢物，其中包括9种花青素。与浅色品种相比，深色品种中所有9种花青素的含量均上调。发现天竺葵素、矢车菊素及其衍生物的不同含量是颜色变异的主要原因。转录组分析表明，颜色差异与花青素生物合成密切相关。花青素结构基因，包括[此处原文缺失具体基因名称]的表达水平与花色深浅一致。这一发现表明，花青素可能是所研究品种间颜色变异的关键因素。在此基础上，选择了两种特殊代谢物作为生物标志物，以辅助菊花颜色选择育种。
