College of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology (Wenzhou Academy of Agricultural Sciences), Wenzhou, Zhejiang, 325006, China.
Southern Zhejiang Key Laboratory of Crop Breeding, Wenzhou Vocational College of Science and Technology (Wenzhou Academy of Agricultural Sciences), Wenzhou, Zhejiang, 325006, China.
BMC Plant Biol. 2023 Jun 27;23(1):342. doi: 10.1186/s12870-023-04356-y.
Auxin/indoleacetic acid (AUX/IAA) genes encoding short-lived proteins participate in AUX signaling transduction and play crucial roles in plant growth and development. Although the AUX/IAA gene family has been identified in many plants, a systematic analysis of AUX/IAA genes in Brassica rapa ssp. rapa has not yet been reported.
We performed a comprehensive genome-wide analysis and found 89 AUX/IAA genes in turnip based on the conserved AUX/IAA domain (pfam02309). Phylogenetic analysis of AUX/IAA genes from turnip, Arabidopsis, and cabbage revealed that these genes cluster into six subgroups (A1, A2, A3, A4, B1, and B2). The motif distribution was also conservative among the internal members of the clade. Enhanced yellow fluorescent protein (EYFP) signals of BrrIAA-EYFPs showed that BrrIAA members functioned as nucleoproteins. Moreover, transcriptional analysis revealed that the expression patterns of AUX/IAA genes in turnip were tissue-dependent. Because orthologs have similar biological functions and interaction networks in plant growth and development, BrrIAA66 in turnip possibly played a role in embryo axis formation, vascular development, lateral root formation, and floral organ development by interacting with BrrARF19 and BrrTIR1.
These results provide a theoretical basis for further investigation of BrrAUX/IAA genes and lay the foundation for functional analysis of BrrIAA66 in turnip.
编码短寿命蛋白的生长素/吲哚乙酸（Auxin/Indoleacetic Acid，AUX/IAA）基因参与 AUX 信号转导，在植物生长发育中发挥着关键作用。尽管在许多植物中已经鉴定出 AUX/IAA 基因家族，但尚未对油菜亚种油菜中的 AUX/IAA 基因进行系统分析。
我们基于保守的 AUX/IAA 结构域（pfam02309），对芜菁进行了全面的基因组-wide 分析，发现了 89 个 AUX/IAA 基因。对芜菁、拟南芥和甘蓝 AUX/IAA 基因的系统发育分析表明，这些基因聚为 6 个亚组（A1、A2、A3、A4、B1 和 B2）。分支内部成员的基序分布也具有保守性。增强型黄色荧光蛋白（Enhanced Yellow Fluorescent Protein，EYFP）信号的 BrrIAA-EYFPs 表明 BrrIAA 成员作为核蛋白发挥作用。此外，转录分析表明，AUX/IAA 基因在芜菁中的表达模式具有组织依赖性。由于在植物生长发育中，同源物具有相似的生物学功能和相互作用网络，因此芜菁中的 BrrIAA66 可能通过与 BrrARF19 和 BrrTIR1 相互作用，在胚胎轴形成、血管发育、侧根形成和花器官发育中发挥作用。
这些结果为进一步研究 BrrAUX/IAA 基因提供了理论基础，并为芜菁中 BrrIAA66 的功能分析奠定了基础。
