College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China.
Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agriculture and Animal Husbandry Science, Lhasa, 850009, China.
Microb Cell Fact. 2023 Jun 27;22(1):116. doi: 10.1186/s12934-023-02119-w.
17β-estradiol (E2) residues exhibit harmful effects both for human and animals and have got global attention of the scientific community. Microbial enzymes are considered as one of the effective strategies having great potential for removal E2 residues from the environment. However, limited literature is available on the removal of E2 from wastewater using short-chain dehydrogenase.
In this study, 17β-estradiol degrading enzyme (17β-HSD-0095) was expressed and purified from Microbacterium sp. MZT7. The optimal pH and temperature for reaction was 7 and 40 °C, respectively. Molecular docking studies have shown that the ARG215 residue form a hydrogen bond with oxygen atom of the substrate E2. Likewise, the point mutation results have revealed that the ARG215 residue play an important role in the E2 degradation by 17β-HSD-0095. In addition, 17β-HSD-0095 could remediate E2 contamination in synthetic livestock wastewater.
These findings offer some fresh perspectives on the molecular process of E2 degradation and the creation of enzyme preparations that can degrade E2.
17β-雌二醇（E2）残留对人类和动物都有有害影响，已引起科学界的全球关注。微生物酶被认为是去除环境中 E2 残留的有效策略之一，具有很大的潜力。然而，利用短链脱氢酶从废水中去除 E2 的相关文献有限。
本研究从微杆菌属 MZT7 中表达和纯化了 17β-雌二醇降解酶（17β-HSD-0095）。反应的最佳 pH 值和温度分别为 7 和 40°C。分子对接研究表明，ARG215 残基与底物 E2 的氧原子形成氢键。同样，点突变结果表明，ARG215 残基在 17β-HSD-0095 降解 E2 中起重要作用。此外，17β-HSD-0095 可以修复合成牲畜废水中的 E2 污染。
这些发现为 E2 降解的分子过程以及可降解 E2 的酶制剂的开发提供了一些新的视角。
