College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China.
Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China.
Environ Res. 2024 Jan 1;240(Pt 2):117513. doi: 10.1016/j.envres.2023.117513. Epub 2023 Oct 27.
Steroidal estrogens residues in the environment can be a serious hazard to humans and animals and has been listed as group 1 carcinogens by World Health Organization (WHO). Microbial degradation is one of the effective strategies for the removal of such contaminants. In this study, a low-temperature degrading bacterial strain (Rhodococcus sp. RCBS9) was isolated from the soil of a dairy farm for 17β-estradiol (E2) degradation. The strain RCBS9 exhibited an efficient degradation potential at low temperatures. To lean how different factors influence E2 degradation, we have found a major role of intracellular enzymes in E2 degradation. Genomic and metabolomic analyses have suggested potential degradation genes and four metabolic pathways. These findings provide valuable strain resources for the low temperature bioremediation of E2 contamination and insights into E2 biodegradation mechanism.
环境中的甾体雌激素残留会对人类和动物造成严重危害，并已被世界卫生组织（WHO）列为 1 类致癌物。微生物降解是去除此类污染物的有效策略之一。在这项研究中，从奶牛场的土壤中分离到一株低温降解菌（Rhodococcus sp. RCBS9），用于 17β-雌二醇（E2）的降解。该菌株 RCBS9 在低温下表现出高效的降解能力。为了研究不同因素对 E2 降解的影响，我们发现细胞内酶在 E2 降解中起主要作用。基因组和代谢组学分析表明了潜在的降解基因和四条代谢途径。这些发现为 E2 污染的低温生物修复提供了有价值的菌株资源，并深入了解了 E2 生物降解机制。
