College of Life Sciences, Qingdao University, Qingdao 266071, China.
Int J Mol Sci. 2023 Jun 16;24(12):10224. doi: 10.3390/ijms241210224.
() is the airborne fungal pathogen that causes powdery mildew disease on hexaploid bread wheat. Calmodulin-binding transcription activators (CAMTAs) regulate plant responses to environments, but their potential functions in the regulation of wheat- interaction remain unknown. In this study, the wheat CAMTA transcription factors TaCAMTA2 and TaCAMTA3 were identified as suppressors of wheat post-penetration resistance against powdery mildew. Transient overexpression of and enhanced the post-penetration susceptibility of wheat to , while knockdown of and expression using transient- or virus-induced gene silencing compromised wheat post-penetration susceptibility to In addition, and were characterized as positive regulators of wheat post-penetration resistance against powdery mildew. Overexpressing and confers wheat post-penetration resistance against , while silencing and enhances wheat post-penetration susceptibility to Importantly, we showed that expressions of and were potentiated by silencing of and Collectively, these results implicated that the genes and contribute to the wheat- compatibility might via negative regulation of and expression.
() 是一种气传真菌病原体，可引起六倍体普通小麦的白粉病。钙调素结合转录激活因子（CAMTAs）调节植物对环境的反应，但它们在调节小麦- 互作中的潜在功能尚不清楚。在这项研究中，鉴定出小麦 CAMTA 转录因子 TaCAMTA2 和 TaCAMTA3 是抑制小麦对白粉病穿透后抗性的因素。和 的瞬时过表达增强了小麦对白粉病的穿透后易感性，而使用瞬时或病毒诱导的基因沉默敲低 和 的表达则削弱了小麦对白粉病的穿透后易感性。此外，和 被表征为小麦对白粉病穿透后抗性的正调控因子。过表达 和 赋予小麦对白粉病的穿透后抗性，而沉默 和 增强了小麦对白粉病的穿透后易感性。重要的是，我们表明通过沉默 和 增强了 和 的表达。总之，这些结果表明，基因 和 可能通过负调控 和 的表达来促进小麦与 的亲和性。
