College of Life Sciences, Qingdao University, Qingdao 266071, China.
Int J Mol Sci. 2023 Jun 16;24(12):10235. doi: 10.3390/ijms241210235.
The waxy cuticle covers a plant's aerial surface and contributes to environmental adaptation in land plants. Although past decades have seen great advances in understanding wax biosynthesis in model plants, the mechanisms underlying wax biosynthesis in crop plants such as bread wheat remain to be elucidated. In this study, wheat MYB transcription factor TaMYB30 was identified as a transcriptional activator positively regulating wheat wax biosynthesis. The knockdown of expression using virus-induced gene silencing led to attenuated wax accumulation, increased water loss rates, and enhanced chlorophyll leaching. Furthermore, and were isolated as essential components of wax biosynthetic machinery in bread wheat. In addition, silencing and resulted in compromised wax biosynthesis and potentiated cuticle permeability. Importantly, we showed that TaMYB30 could directly bind to the promoter regions of and genes by recognizing the and cis-elements, and activate their expressions. These results collectively demonstrated that TaMYB30 positively regulates wheat wax biosynthesis presumably via the transcriptional activation of and .
蜡质表皮覆盖在植物的气生表面，有助于陆生植物适应环境。尽管过去几十年在理解模式植物的蜡质生物合成方面取得了重大进展，但作物如面包小麦中蜡质生物合成的机制仍有待阐明。在这项研究中，小麦 MYB 转录因子 TaMYB30 被鉴定为正向调控小麦蜡质生物合成的转录激活因子。利用病毒诱导的基因沉默技术敲低表达导致蜡质积累减弱、水分损失率增加和叶绿素浸出加剧。此外， 和 被分离为面包小麦蜡质生物合成机制的必需成分。此外，沉默 和 导致蜡质生物合成受损，角质层通透性增强。重要的是，我们通过识别 和 顺式元件表明 TaMYB30 可以直接结合到 和 基因的启动子区域，并激活它们的表达。这些结果共同表明，TaMYB30 可能通过对 和 基因的转录激活正向调控小麦蜡质生物合成。
