抗原诱导的白细胞介素-1受体拮抗剂产生可区分活动性和潜伏性结核感染。

Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection.

作者信息

Sanchez Cesar, Jaramillo-Valverde Luis, Capristano Silvia, Solis Gilmer, Soto Alonso, Valdivia-Silva Julio, Poterico Julio A, Guio Heinner

机构信息

Laboratorio de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima 15046, Peru.

Escuela de Posgrado, Universidad Cesar Vallejo, Lima 15314, Peru.

出版信息

Microorganisms. 2023 May 25;11(6):1385. doi: 10.3390/microorganisms11061385.

The IGRA (Interferon Gamma Release Assays) test is currently the standard specific test for infection status. However, a positive test cannot distinguish between active tuberculosis disease (ATBD) and latent tuberculosis infection (LTBI). Developing a test with this characteristic is needed. We conducted longitudinal studies to identify a combination of antigen peptides and cytokines to discriminate between ATBD and LTBI. We studied 54 patients with ATBD disease and 51 with LTBI infection. Cell culture supernatant from cells stimulated with overlapping novel peptides and 40 cytokines/chemokines were analyzed using the Luminex technology. To summarize longitudinal measurements of analyte levels, we calculated the area under the curve (AUC). Our results indicate that in vitro cell stimulation with a novel combination of peptides (Rv0849-12, Rv2031c-14, Rv2031c-5, and Rv2693-06) and IL-1RA detection in culture supernatants can discriminate between LTBI and ATBD.

γ-干扰素释放试验（IGRA）目前是检测感染状况的标准特异性检测方法。然而，检测结果呈阳性无法区分活动性结核病（ATBD）和潜伏性结核感染（LTBI）。因此需要开发一种具有此特性的检测方法。我们开展了纵向研究，以确定能区分ATBD和LTBI的抗原肽和细胞因子组合。我们研究了54例ATBD患者和51例LTBI感染者。使用Luminex技术分析了用重叠新型肽和40种细胞因子/趋化因子刺激的细胞培养上清液。为了总结分析物水平的纵向测量结果，我们计算了曲线下面积（AUC）。我们的结果表明，用新型肽组合（Rv0849-12、Rv2031c-14、Rv2031c-5和Rv2693-06）进行体外细胞刺激，并检测培养上清液中的白细胞介素-1受体拮抗剂（IL-1RA），可以区分LTBI和ATBD。