Liu Yankun, Yang Xuewei, Zhu Meichen, Bai Na, Wang Wenjie, Yang Jinkui
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, School of Life Science, Yunnan University, Kunming 650032, China.
Microorganisms. 2023 Jun 19;11(6):1612. doi: 10.3390/microorganisms11061612.
Multidrug resistance (Mdr) proteins are critical proteins for maintenance of drug resistance in fungi. Mdr1 has been extensively studied in ; its role in other fungi is largely unknown. In this study, we identified a homologous protein of Mdr (AoMdr1) in the nematode-trapping (NT) fungus . It was found that the deletion of resulted in a significant reduction in the number of hyphal septa and nuclei as well as increased sensitivity to fluconazole and resistance to hyperosmotic stress and SDS. The deletion of also led to a remarkable increase in the numbers of traps and mycelial loops in the traps. Notably, AoMdr1 was able to regulate mycelial fusion under low-nutrient conditions, but not under nutrient-rich conditions. AoMdr1 was also involved in secondary metabolism, and its deletion caused an increase in arthrobotrisins (specific compounds produced by NT fungi). These results suggest that AoMdr1 plays a crucial role in the fluconazole resistance, mycelial fusion, conidiation, trap formation, and secondary metabolism of . Our study contributes to the understanding of the critical role of Mdr proteins in mycelial growth and the development of NT fungi.
多药耐药（Mdr）蛋白是真菌中维持耐药性的关键蛋白。Mdr1已在[具体物种]中得到广泛研究；其在其他真菌中的作用在很大程度上尚不清楚。在本研究中，我们在捕食线虫真菌[具体真菌名称]中鉴定出一种Mdr的同源蛋白（AoMdr1）。研究发现，AoMdr1的缺失导致菌丝隔膜和细胞核数量显著减少，同时对氟康唑的敏感性增加，对高渗胁迫和十二烷基硫酸钠的抗性增强。AoMdr1的缺失还导致捕食器和捕食器中菌丝环的数量显著增加。值得注意的是，AoMdr1能够在低营养条件下调节菌丝融合，但在营养丰富条件下则不能。AoMdr1还参与次级代谢，其缺失导致节丛孢菌素（捕食线虫真菌产生的特定化合物）增加。这些结果表明，AoMdr1在[具体真菌名称]的氟康唑抗性、菌丝融合、分生孢子形成、捕食器形成和次级代谢中起关键作用。我们的研究有助于理解Mdr蛋白在捕食线虫真菌菌丝生长和发育中的关键作用。
