State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China.
Molecules. 2023 Jun 7;28(12):4615. doi: 10.3390/molecules28124615.
Glucose oxidase (GOD) is an oxidoreductase that catalyzes the aerobic oxidation of glucose into hydrogen peroxide (HO) and gluconic acid, which has been widely used in industrial raw materials production, biosensors and cancer treatment. However, natural GOD bears intrinsic disadvantages, such as poor stability and a complex purification process, which undoubtedly restricts its biomedical applications. Fortunately, several artificial nanomaterials have been recently discovered with a GOD-like activity and their catalytic efficiency toward glucose oxidation can be finely optimized for diverse biomedical applications in biosensing and disease treatments. In view of the notable progress of GOD-mimicking nanozymes, this review systematically summarizes the representative GOD-mimicking nanomaterials for the first time and depicts their proposed catalytic mechanisms. We then introduce the efficient modulation strategy to improve the catalytic activity of existing GOD-mimicking nanomaterials. Finally, the potential biomedical applications in glucose detection, DNA bioanalysis and cancer treatment are highlighted. We believe that the development of nanomaterials with a GOD-like activity will expand the application range of GOD-based systems and lead to new opportunities of GOD-mimicking nanomaterials for various biomedical applications.
葡萄糖氧化酶（GOD）是一种氧化还原酶，能够催化葡萄糖的有氧氧化为过氧化氢（HO）和葡萄糖酸，已广泛应用于工业原料生产、生物传感器和癌症治疗等领域。然而，天然 GOD 存在固有缺点，如稳定性差和纯化过程复杂，这无疑限制了其在生物医学中的应用。幸运的是，最近发现了几种具有 GOD 样活性的人工纳米材料，其对葡萄糖氧化的催化效率可以针对生物传感和疾病治疗等各种生物医学应用进行精细优化。鉴于 GOD 模拟纳米酶的显著进展，本综述首次系统地总结了具有代表性的 GOD 模拟纳米材料，并描述了它们的拟议催化机制。然后，我们介绍了提高现有 GOD 模拟纳米材料催化活性的有效调控策略。最后，强调了在葡萄糖检测、DNA 生物分析和癌症治疗等方面的潜在生物医学应用。我们相信，具有 GOD 样活性的纳米材料的发展将扩大基于 GOD 的系统的应用范围，并为各种生物医学应用的 GOD 模拟纳米材料带来新的机遇。
