Department of Wood, Cellulose and Paper, University of Guadalajara, CUCEI, Road Ing. Ramón Padilla Sánchez 2100, Las Agujas, Zapopan 45200, Jalisco, Mexico.
Group of Research and Development in Science Applied to Biological Resources, Salesian Polytechnic University, 12 de Octubre Avenue N2422 and Wilson, Quito 170109, Ecuador.
Molecules. 2023 Jun 12;28(12):4719. doi: 10.3390/molecules28124719.
is a plant that is located in various regions of Mexico and is used in a traditional way against illness. Essential oils (EOs) were obtained from leaves and the chemical composition was evaluated by gas chromatography-mass spectrometry (GC-MS). The antioxidant effect of the oil was assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by Trolox Equivalent Antioxidant Capacity (TEAC). In vitro antibacterial activity against and was determined using a broth microdilution assay and thin layer chromatography-direct bioautography (TLC-DB) to identify active antibacterial compounds. The EOs analysis showed 21 compounds, 99% terpenes, and 96% oxygenated monoterpenes, with -piperitone epoxide (46%), -piperitone epoxide (22%), and piperitenone oxide (11%) as more abundant compounds. Likewise, EOs showed an antioxidant activity of DPPH = 82%, with 50% free radical scavenging (IC) = 7 mg/mL and TEAC = 0.005, an antibacterial effect against of 73% inhibition, and 81% over at dose of 100 µL of undiluted crude oil. The TLC-DB assay showed that the most active compounds were derived from piperitone. The comparison with other studies on shows variability in the compounds and their abundances, which can be attributed to climatic factors and the maturity of plants with similar antioxidant and antibacterial activities.
是一种生长在墨西哥不同地区的植物，传统上用于治疗疾病。从叶子中提取精油（EOs），并通过气相色谱-质谱联用（GC-MS）评估其化学成分。通过 2,2-二苯基-1-苦基肼基（DPPH）和 Trolox 等效抗氧化能力（TEAC）测定油的抗氧化活性。使用肉汤微量稀释法和薄层色谱-直接生物自显影法（TLC-DB）测定对 和 的体外抗菌活性，以鉴定活性抗菌化合物。EOs 分析表明含有 21 种化合物，99%为萜类化合物，96%为含氧单萜类化合物，其中 -派烯环氧化物（46%）、-派烯环氧化物（22%）和胡椒酮氧化物（11%）为更丰富的化合物。同样，EOs 对 DPPH 的抗氧化活性为 82%，50%自由基清除率（IC）=7mg/mL，TEAC=0.005，对 的抗菌作用为 73%抑制，对 的抗菌作用为 81%，剂量为 100μL 未稀释粗油。TLC-DB 分析表明，最活跃的化合物来自派烯。与其他关于 的研究相比，化合物及其丰度存在差异，这可能归因于气候因素和具有相似抗氧化和抗菌活性的植物的成熟度。
