Univ. Lille, Inserm, CHU Lille, U1286-INFINITE-Institute for Translational Research in Inflammation, F-59000 Lille, France.
CHU Lille, Division of Gastroenterology, Hepatology and Nutrition, Department of Paediatrics, Jeanne de Flandre Children's Hospital, F-59000 Lille, France.
Nutrients. 2023 Jun 16;15(12):2771. doi: 10.3390/nu15122771.
An inadequate perinatal nutritional environment can alter the maturation of the intestinal barrier and promote long-term pathologies such as metabolic syndrome or chronic intestinal diseases. The intestinal microbiota seems to play a determining role in the development of the intestinal barrier. In the present study, we investigated the impact of consuming an early postnatal prebiotic fiber (PF) on growth, intestinal morphology and the microbiota at weaning in postnatal-growth-restricted mice (PNGR).
Large litters (15 pups/mother) were generated from FVB/NRj mice to induce PNGR at postnatal day 4 (PN4) and compared to control litters (CTRL, 8 pups/mother). PF (a resistant dextrin) or water was orally administered once daily to the pups from PN8 to PN20 (3.5 g/kg/day). Intestinal morphology was evaluated at weaning (PN21) using the ileum and colon. Microbial colonization and short-chain fatty acid (SCFA) production were investigated using fecal and cecal contents.
At weaning, the PNGR mice showed decreased body weight and ileal crypt depth compared to the CTRL. The PNGR microbiota was associated with decreased proportions of the Lachnospiraceae and Oscillospiraceae families and the presence of the Akkermansia family and Enterococcus genus compared to the CTRL pups. The propionate concentrations were also increased with PNGR. While PF supplementation did not impact intestinal morphology in the PNGR pups, the proportions of the Bacteroides and Parabacteroides genera were enriched, but the proportion of the Proteobacteria phylum was reduced. In the CTRL pups, the Akkermansia genus (Verrucomicrobiota phylum) was present in the PF-supplemented CTRL pups compared to the water-supplemented ones.
PNGR alters intestinal crypt maturation in the ileum at weaning and gut microbiota colonization. Our data support the notion that PF supplementation might improve gut microbiota establishment during the early postnatal period.
围产期营养环境不足会改变肠道屏障的成熟度，并促进代谢综合征或慢性肠道疾病等长期病理变化。肠道微生物群似乎在肠道屏障的发育中起决定性作用。在本研究中，我们研究了在出生后生长受限（PNGR）的小鼠中，早期产后益生元纤维（PF）的摄入对断奶时生长、肠道形态和微生物群的影响。
从 FVB/NRj 小鼠中产生大窝（15 只/只），在产后第 4 天（PN4）诱导 PNGR，并与对照窝（CTRL，8 只/只）进行比较。从 PN8 到 PN20 每天一次向幼鼠口服 PF（抗性糊精）或水（3.5 g/kg/天）。在断奶时（PN21）使用回肠和结肠评估肠道形态。使用粪便和盲肠内容物研究微生物定植和短链脂肪酸（SCFA）的产生。
在断奶时，与 CTRL 相比，PNGR 小鼠的体重和回肠隐窝深度降低。与 CTRL 相比，PNGR 微生物群与lachnospiraceae 和 Oscillospiraceae 家族的比例降低以及 Akkermansia 家族和 Enterococcus 属的存在有关。丙酸浓度也随着 PNGR 的增加而增加。虽然 PF 补充剂对 PNGR 幼鼠的肠道形态没有影响，但Bacteroides 和 Parabacteroides 属的比例增加，而 Proteobacteria 门的比例减少。在 CTRL 幼鼠中，阿克曼氏菌属（Verrucomicrobiota 门）存在于 PF 补充的 CTRL 幼鼠中，而不存在于水补充的幼鼠中。
PNGR 改变了断奶时回肠隐窝的成熟度和肠道微生物群定植。我们的数据支持这样一种观点，即 PF 补充可能会改善早期产后肠道微生物群的建立。
