Babacan Ebru Yuce, Zheleva-Dimitrova Dimitrina, Gevrenova Reneta, Bouyahya Abdelhakim, Balos Mehmet Maruf, Cakilcioglu Ugur, Sinan Kouadio Ibrahime, Zengin Gokhan
Pertek Sakine Genç Vocational School, Munzur University, Pertek, Tunceli 62500, Turkey.
Department of Pharmacognosy, Faculty of Pharmacy, Medical University-Sofia, 1000 Sofia, Bulgaria.
Plants (Basel). 2023 Jun 8;12(12):2252. doi: 10.3390/plants12122252.
The study aimed at the metabolite profiling and evaluation of antioxidant and enzyme inhibitory properties of methanol extracts from flowers, leaves, and tubers of unexplored (Banks & Sol.) Kuntze and (Blume) Schott (Araceae). A total of 83 metabolites, including 19 phenolic acids, 46 flavonoids, 11 amino, and 7 fatty acids were identified by UHPLC-HRMS in the studied extracts for the first time. flower and leaf extracts had the highest total phenolic and flavonoid contents (50.82 ± 0.71 mg GAE/g and 65.08 ± 0.38 RE/g, respectively). Significant radical scavenging activity (32.20 ± 1.26 and 54.34 ± 0.53 mg TE/g for DPPH and ABTS) and reducing power (88.27 ± 1.49 and 33.13 ± 0.68 mg TE/g for CUPRAC and FRAP) were observed in leaf extracts. flowers showed the maximum anticholinesterase activity (2.72 ± 0.03 mg GALAE/g). leaves and tubers exhibited the highest inhibition towards α-glucosidase (0.99 ± 0.02 ACAE/g) and tirosinase (50.73 ± 2.29 mg KAE/g), respectively. A multivariate analysis revealed that -hydroxycinnamoylglycosyl--flavonoid glycosides mostly accounted for the discrimination of both species. Thus, and can be considered as potential candidates for designing functional ingredients in the pharmaceutical and nutraceutical industries.
本研究旨在对未被探索的犁头尖属（Banks & Sol.）Kuntze和半夏属（Blume）Schott（天南星科）的花、叶和块茎的甲醇提取物进行代谢物谱分析，并评估其抗氧化和酶抑制特性。通过超高效液相色谱-高分辨质谱（UHPLC-HRMS）首次在研究的提取物中鉴定出总共83种代谢物，包括19种酚酸、46种黄酮类化合物、11种氨基酸和7种脂肪酸。花和叶提取物的总酚和黄酮含量最高（分别为50.82±0.71毫克没食子酸当量/克和65.08±0.38毫克芦丁当量/克）。叶提取物表现出显著的自由基清除活性（DPPH和ABTS分别为32.20±1.26和54.34±0.53毫克 Trolox当量/克）和还原能力（CUPRAC和FRAP分别为88.27±1.49和33.13±0.68毫克 Trolox当量/克）。花表现出最大的抗胆碱酯酶活性（2.72±0.03毫克加兰他敏当量/克）。叶和块茎分别对α-葡萄糖苷酶（0.99±0.02阿卡波糖当量/克）和酪氨酸酶（50.73±2.29毫克曲酸当量/克）表现出最高的抑制作用。多变量分析表明，对香豆酰基糖苷-黄酮糖苷主要导致了两种植物的区分。因此，犁头尖属和半夏属可被视为制药和营养保健品行业设计功能性成分的潜在候选植物。
