Vidya Chirappurathu Sukumaran Nair, Shetty Rajpal, Bokor Boris, Fialová Ivana, Luxová Miroslava, Jašková Katarína, Vaculík Marek
Institute of Botany, Plant Science and Biodiversity Centre, Slovak Academy of Sciences, Dúbravská cesta 14, SK-845 23 Bratislava, Slovakia.
Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynska dolina B2, Ilkovicova 6, SK-842 15 Bratislava, Slovakia.
Plants (Basel). 2023 Jun 19;12(12):2368. doi: 10.3390/plants12122368.
A study was conducted to further develop our understanding of antimony (Sb) uptake in plants. Unlike other metal(loid)s, such as silicon (Si), the mechanisms of Sb uptake are not well understood. However, SbIII is thought to enter the cell via aquaglyceroporins. We investigated if the channel protein Lsi1, which aids in Si uptake, also plays a role in Sb uptake. Seedlings of WT sorghum, with normal silicon accumulation, and its mutant (), with low silicon accumulation, were grown in Hoagland solution for 22 days in the growth chamber under controlled conditions. Control, Sb (10 mg Sb L), Si (1mM) and Sb + Si (10 mg Sb L + 1 mM Si) were the treatments. After 22 days, root and shoot biomass, the concentration of elements in root and shoot tissues, lipid peroxidation and ascorbate levels, and relative expression of were determined. When mutant plants were exposed to Sb, they showed almost no toxicity symptoms compared to WT plants, indicating that Sb was not toxic to mutant plants. On the other hand, WT plants had decreased root and shoot biomass, increased MDA content and increased Sb uptake compared to mutant plants. In the presence of Sb, we also found that was downregulated in the roots of WT plants. The results of this experiment support the role of Lsi1 in Sb uptake in sorghum plants.
开展了一项研究以进一步加深我们对植物中锑（Sb）吸收的理解。与其他金属（类金属）如硅（Si）不同，锑的吸收机制尚未得到很好的理解。然而，三价锑（SbIII）被认为是通过水甘油通道蛋白进入细胞的。我们研究了有助于硅吸收的通道蛋白Lsi1是否也在锑的吸收中发挥作用。将具有正常硅积累的野生型高粱幼苗及其硅积累量低的突变体在可控条件下于生长室中的霍格兰溶液中培养22天。处理包括对照、锑（10毫克锑/升）、硅（1毫摩尔）以及锑+硅（10毫克锑/升+1毫摩尔硅）。22天后，测定根和地上部生物量、根和地上部组织中元素的浓度、脂质过氧化和抗坏血酸水平以及相关基因的相对表达。当突变体植物暴露于锑时，与野生型植物相比，它们几乎没有表现出毒性症状，这表明锑对突变体植物无毒。另一方面，与突变体植物相比，野生型植物的根和地上部生物量减少、丙二醛（MDA）含量增加且锑吸收增加。在有锑存在的情况下，我们还发现野生型植物根中的相关基因表达下调。该实验结果支持了Lsi1在高粱植物锑吸收中的作用。
