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基于聚（ε-己内酯）和用寡聚/聚（谷氨酸）改性的氧化石墨烯的复合材料作为具有骨传导特性的生物材料。

Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties.

作者信息

Solomakha Olga, Stepanova Mariia, Gofman Iosif, Nashchekina Yulia, Rabchinskii Maxim, Nashchekin Alexey, Lavrentieva Antonina, Korzhikova-Vlakh Evgenia

机构信息

Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg 199004, Russia.

Institute of Cytology, Russian Academy of Sciences, St. Petersburg 194064, Russia.

出版信息

Polymers (Basel). 2023 Jun 17;15(12):2714. doi: 10.3390/polym15122714.

DOI:10.3390/polym15122714
PMID:37376360
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10301562/
Abstract

The development of new biodegradable biomaterials with osteoconductive properties for bone tissue regeneration is one of the urgent tasks of modern medicine. In this study, we proposed the pathway for graphene oxide (GO) modification with oligo/poly(glutamic acid) (oligo/poly(Glu)) possessing osteoconductive properties. The modification was confirmed by a number of methods such as Fourier-transform infrared spectroscopy, quantitative amino acid HPLC analysis, thermogravimetric analysis, scanning electron microscopy, and dynamic and electrophoretic light scattering. Modified GO was used as a filler for poly(ε-caprolactone) (PCL) in the fabrication of composite films. The mechanical properties of the biocomposites were compared with those obtained for the PCL/GO composites. An 18-27% increase in elastic modulus was found for all composites containing modified GO. No significant cytotoxicity of the GO and its derivatives in human osteosarcoma cells (MG-63) was revealed. Moreover, the developed composites stimulated the proliferation of human mesenchymal stem cells (hMSCs) adhered to the surface of the films in comparison with unfilled PCL material. The osteoconductive properties of the PCL-based composites filled with GO modified with oligo/poly(Glu) were confirmed via alkaline phosphatase assay as well as calcein and alizarin red S staining after osteogenic differentiation of hMSC in vitro.

摘要

开发具有骨传导特性的新型可生物降解生物材料用于骨组织再生是现代医学的紧迫任务之一。在本研究中，我们提出了用具有骨传导特性的寡聚/聚（谷氨酸）（oligo/poly(Glu)）修饰氧化石墨烯（GO）的途径。通过多种方法证实了这种修饰，如傅里叶变换红外光谱、定量氨基酸高效液相色谱分析、热重分析、扫描电子显微镜以及动态和电泳光散射。在复合膜制备中，将修饰后的GO用作聚（ε-己内酯）（PCL）的填料。将生物复合材料的力学性能与PCL/GO复合材料的力学性能进行了比较。发现所有含有修饰后GO的复合材料的弹性模量提高了18 - 27%。未发现GO及其衍生物对人骨肉瘤细胞（MG-63）有明显的细胞毒性。此外，与未填充PCL材料相比，所开发的复合材料刺激了粘附在膜表面的人间充质干细胞（hMSCs）的增殖。通过碱性磷酸酶测定以及hMSC体外成骨分化后的钙黄绿素和茜素红S染色，证实了用oligo/poly(Glu)修饰的GO填充的基于PCL的复合材料的骨传导特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/acf7c49e1d17/polymers-15-02714-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/f8f0a6dc0360/polymers-15-02714-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/53f38804b24e/polymers-15-02714-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/884d8a8febb0/polymers-15-02714-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/810954779558/polymers-15-02714-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/018a76b06582/polymers-15-02714-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/07b41c1662aa/polymers-15-02714-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/2d592dfdc39c/polymers-15-02714-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/f44e55debffe/polymers-15-02714-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/513366b01ece/polymers-15-02714-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/acf7c49e1d17/polymers-15-02714-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/f8f0a6dc0360/polymers-15-02714-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/53f38804b24e/polymers-15-02714-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/884d8a8febb0/polymers-15-02714-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/810954779558/polymers-15-02714-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/018a76b06582/polymers-15-02714-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/07b41c1662aa/polymers-15-02714-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/2d592dfdc39c/polymers-15-02714-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/f44e55debffe/polymers-15-02714-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/513366b01ece/polymers-15-02714-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab52/10301562/acf7c49e1d17/polymers-15-02714-g010.jpg

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