Laboratorio de Biotecnología, Universidad Católica de Cuenca, Cuenca 010107, Ecuador.
Hospital Luis Vernaza, Guayaquil 090306, Ecuador.
Viruses. 2023 Jun 19;15(6):1393. doi: 10.3390/v15061393.
Human Papillomavirus (HPV) infection is associated with intraepithelial neoplasia and cervical cancer (CC). Ecuador has a high prevalence of cervical cancer, with more than 1600 new cases diagnosed annually. This study aimed to analyze oncogenes E6 and E7 of HPV16 in samples collected from women with cancerous and precancerous cervical lesions from the Ecuadorian coast. Twenty-nine women, including six with ASCUS, three with LSIL, thirteen with HSIL, and seven with Cacu, were analyzed. The most common SNPs were E6 350G or L83V (82.6%) and E6 145T/286A/289G/335T/350G or Q14H/F78Y/L83V (17.4%). Both variants are reported to be associated with an increased risk of cervical cancer in worldwide studies. In contrast, all E7 genes have conserved amino-acid positions. Phylogenetic trees showed the circulation of the D (26.1%) and A (73.9) lineages. The frequency of D was higher than that reported in other comparable studies in Ecuador and Latin America, and may be related to the ethnic composition of the studied populations. This study contributes to the characterization of the potential risk factors for cervical carcinogenesis associated with Ecuadorian women infected with HPV16.
人乳头瘤病毒（HPV）感染与上皮内瘤变和宫颈癌（CC）有关。厄瓜多尔宫颈癌的发病率很高，每年诊断出超过 1600 例新病例。本研究旨在分析厄瓜多尔沿海地区患有宫颈癌前病变和癌症的女性样本中 HPV16 的致癌基因 E6 和 E7。对 29 名女性进行了分析，包括 6 名 ASCUS、3 名 LSIL、13 名 HSIL 和 7 名 Cacu。最常见的 SNP 是 E6 350G 或 L83V（82.6%）和 E6 145T/286A/289G/335T/350G 或 Q14H/F78Y/L83V（17.4%）。这两种变体都与全球范围内宫颈癌风险增加有关。相比之下，所有 E7 基因都具有保守的氨基酸位置。系统发育树显示 D（26.1%）和 A（73.9%）谱系的循环。D 的频率高于在厄瓜多尔和拉丁美洲其他可比研究中的报告频率，这可能与研究人群的种族构成有关。本研究有助于确定与感染 HPV16 的厄瓜多尔妇女宫颈癌发生相关的潜在危险因素。
Zotero 插件