Chomová Natália, Pavloková Sylvie, Sondorová Miriam, Mudroňová Dagmar, Fečkaninová Adriána, Popelka Peter, Koščová Jana, Žitňan Rudolf, Franc Aleš
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Košice, Slovakia.
Department of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, Brno, Czechia.
Front Vet Sci. 2023 Jun 12;10:1196884. doi: 10.3389/fvets.2023.1196884. eCollection 2023.
Due to the intensification of fish farming and the associated spread of antimicrobial resistance among animals and humans, it is necessary to discover new alternatives in the therapy and prophylaxis of diseases. Probiotics appear to be promising candidates because of their ability to stimulate immune responses and suppress the growth of pathogens.
The aim of this study was to prepare fish feed mixtures with various compositions and, based on their physical characteristics (sphericity, flow rate, density, hardness, friability, and loss on drying), choose the most suitable one for coating with the selected probiotic strain R2 Biocenol™ CCM 8674 (new nom. ). The probiotic strain was examined through sequence analysis for the presence of plantaricin- related genes. An invented coating technology based on a dry coating with colloidal silica followed by starch hydrogel containing was applied to pellets and tested for the viability of probiotics during an 11-month period at different temperatures (4°C and 22°C). The release kinetics of probiotics in artificial gastric juice and in water (pH = 2 and pH = 7) were also determined. Chemical and nutritional analyses were conducted for comparison of the quality of the control and coated pellets.
The results showed a gradual and sufficient release of probiotics for a 24-hour period, from 10 CFU at 10 mi up to 10 at the end of measurement in both environments. The number of living probiotic bacteria was stable during the whole storage period at 4°C (10), and no significant decrease in living probiotic bacteria was observed. Sanger sequencing revealed the presence of plantaricin A and plantaricin EF. Chemical analysis revealed an increase in multiple nutrients compared to the uncoated cores. These findings disclose that the invented coating method with a selected probiotic strain improved nutrient composition and did not worsen any of the physical characteristics of pellets. Applied probiotics are also gradually released into the environment and have a high survival rate when stored at 4°C for a long period of time. The outputs of this study confirm the potential of prepared and tested probiotic fish mixtures for future use in experiments and in fish farms for the prevention of infectious diseases.
由于养鱼业的集约化以及动物和人类中抗菌素耐药性的相关传播，有必要在疾病治疗和预防方面寻找新的替代方法。益生菌因其能够刺激免疫反应和抑制病原体生长，似乎是很有前景的候选者。
本研究的目的是制备具有不同成分的鱼饲料混合物，并根据其物理特性（球形度、流速、密度、硬度、脆碎度和干燥失重），选择最适合用选定的益生菌菌株R2 Biocenol™ CCM 8674（新命名）进行包衣的混合物。通过序列分析检查益生菌菌株中是否存在与植物乳杆菌素相关的基因。一种基于用胶体二氧化硅进行干包衣，然后用含有的淀粉水凝胶进行包衣的发明技术应用于颗粒，并在不同温度（4°C和22°C）下的11个月期间测试益生菌的活力。还测定了益生菌在人工胃液和水（pH = 2和pH = 7）中的释放动力学。进行化学和营养分析以比较对照颗粒和包衣颗粒的质量。
结果表明，在两种环境中，益生菌在24小时内逐渐充分释放，从10分钟时的10 CFU到测量结束时的10 。在4°C的整个储存期内，活益生菌数量稳定（10），未观察到活益生菌数量有显著下降。桑格测序显示存在植物乳杆菌素A和植物乳杆菌素EF。化学分析表明，与未包衣的核心相比，多种营养素有所增加。这些发现表明，用选定的益生菌菌株进行的发明包衣方法改善了营养成分，且没有使颗粒的任何物理特性变差。应用的益生菌也逐渐释放到环境中，并且在4°C下长期储存时具有较高的存活率。本研究的结果证实了制备和测试的益生菌鱼混合物在未来实验和养鱼场中用于预防传染病的潜力。
